cerevisiae strains, systematic nomenclature,
data sheets, deletions,
According to the different projects the collection consists
of gene deletions in different genetic backgrounds. We deliver the wild
strains and the deletion
strains. The deletion strains are ordered by the systematic nomenclature
of the deleted genes. This nomenclature consists of a three upper case
letters, a three digit numerical code, and a lower case letter indicating
the direction of the open reading frame on the chromosome.
e.g.: YAL001c and YBR003w
||encoded by the "Crick" strand
||encoded by the "Watson" strand
If your gene of choice is available in the collection
you will find the systematic name in the overview of the corresponding
chromosome. For further information follow the hyperlink to the data sheet
corresponding to the open reading frame. The sheet contains all information
for the strains available corresponding to the given open reading frame.
You need the systematic name of your gene of interest to find the corresponding
deletion strain(s) in the EUROSCARF collection. The collection is ordered
by the corresponding chromosomes. A list
of synonyms is offered to find the sythematic name corresponding to
your gene of interest.
For each orf deleted a data sheet exisists listing all corresponding
yeast strains and plasmids available. To find the link to the data sheet
you have to contact first the chromosome
overview in the collection index. For each individual chromosome you
find a list of orfs for which strains are available. This list is ordered
by the systematic nomenclature. If you do not know the systematic name
of your gene of interest please try to find the name in the synonyms
The first entry of the genotype field of each strain
gives the reference
strain from which the strain has been derived. This is followed by
the genotype itself.
Description of deletion
The deletions constructed in the three projects aimed to
delete at least most of the open reading frame. Most times the whole reading
frame has been deleted in order to avoid influences on the expression of
the neighboring gene.
The deletions are described as follows:
eg. YBL054w(4, 1572)::kanMX4
Which means that the open reading frame YBL054w has been
replaced by kanMX4, a kannamycin resistance gene mediating G418 resistance
to the Saccharomyces cerevisiae strain. The open reading frame is
deleted from nucleotide 4 to 1572 corresponding to the A in the translation
start codon AUG. This exact information on the coordinates of the deleted
regions is available for most strains in the collection.
In addition to the strains corresponding plasmids are collected.
One set of plasmids contains the cognate open reading
frames for complementation of the deletions.
The other set contains plasmids with the cloned deletion
The information on the availability of plasmids also
provided in the data sheets to the strains.
You need the systematic name of your gene of interest
to find the corresponding plasmids in the collection.
Other plasmids for yeast genome analysis constructed
in the European functional analysis project are also available. Three series
of plasmids that have been constucted for mediating tetracycline
dependent expression of gene products in Saccharomyces cerevisiae.
These plasmids are delivered to the scientific community by EUROSCARF.