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Saccharomyces cerevisiae strains   According to the different projects the collection consists of gene deletions in different genetic backgrounds. We deliver the wild type reference strains and the deletion strains. The deletion strains are ordered by the systematic nomenclature of the deleted genes. This nomenclature consists of a three upper case letters, a three digit numerical code, and a lower case letter indicating the direction of the open reading frame on the chromosome.   e.g.: YAL001c and YBR003w

Y	yeast	Y	yeast
A	chromosome I	B	chromosome II
L	left arm	R	right arm
001	chromosomal position	003	chromosomal position
c	encoded by the "Crick" strand	w	encoded by the "Watson" strand

If your gene of choice is available in the collection you will find the systematic name in the overview of the corresponding chromosome. For further information follow the hyperlink to the data sheet corresponding to the open reading frame. The sheet contains all information for the strains available corresponding to the given open reading frame. You need the systematic name of your gene of interest to find the corresponding deletion strain(s) in the EUROSCARF collection. The collection is ordered by the corresponding chromosomes. A list of synonyms is offered to find the sythematic name corresponding to your gene of interest.

Data Sheets    For each orf deleted a data sheet exisists listing all corresponding yeast strains and plasmids available. To find the link to the data sheet you have to contact first the chromosome overview in the collection index. For each individual chromosome you find a list of orfs for which strains are available. This list is ordered by the systematic nomenclature. If you do not know the systematic name of your gene of interest please try to find the name in the synonyms list. 
The first entry of the genotype field of each strain gives the reference strain from which the strain has been derived. This is followed by the genotype itself.

Description of deletion 

  The deletions constructed in the three projects aimed to delete at least most of the open reading frame. Most times the whole reading frame has been deleted in order to avoid influences on the expression of the neighboring gene.   The deletions are described as follows:  eg. YBL054w(4, 1572)::kanMX4 Which means that the open reading frame YBL054w has been replaced by kanMX4, a kannamycin resistance gene mediating G418 resistance to the Saccharomyces cerevisiae strain. The open reading frame is deleted from nucleotide 4 to 1572 corresponding to the A in the translation start codon AUG. This exact information on the coordinates of the deleted regions is available for most strains in the collection.   Plasmids   In addition to the strains corresponding plasmids are collected.
One set of plasmids contains the cognate open reading frames for complementation of the deletions. 
The other set contains plasmids with the cloned deletion cassettes. 
The information on the availability of plasmids also provided in the data sheets to the strains. 
You need the systematic name of your gene of interest to find the corresponding plasmids in the collection.
Other plasmids for yeast genome analysis constructed in the European functional analysis project are also available. Three series of plasmids that have been constucted for mediating tetracycline dependent expression of gene products in Saccharomyces cerevisiae. These plasmids are delivered to the scientific community by EUROSCARF. 

SGD

YPD