German Functional Analysis Project,
I, Worldwide Gene Deletion Project (EUROFAN II)
German yeast functional analysis project
During the german yeast functional analysis project
(1994-1997) supported by the BMBF (Bundesministerium für Bildung und
Forschung) 325 genes have been deleted. A collaboration of 15 laboratories
worked on the deletion of the genes and on the functional analysis of the
deletion strains (Entian et al.: Mol Gen Genet 262 (1999) 683-702).
All deletions were done in the strain CEN.PK2.
Due to the early start of the project most gene deletions were made by
replacing the gene of interest with an auxotrophic marker. About one third
of the deletions were carried out by PCR techniques. For this reason there
are a variety of transformation markers with which the genes were replaced.
From this project EUROSCARF harbours haploid strains of both mating types
and the heterozygous diploid strains. Only the heterozygous diploids are
available for essential genes. Strains contributed by this project can
be identified by a preceeding B in the accession number followed by a 4
digit numerical code and a terminal letter.
This collaboration of 115 groups was supported by the European
Union. The project started in 1/96 and 800 open reading frames were deleted
and analysed. Each participating laboratory took over the responsibility
to delete and preliminary analyse six genes (sixpack). The deletion strategy
had been worked out by Wach et al. (Yeast 10 (1994) 1793-1808).
The strategy is based on PCR fragments to delete the respective open reading
frames. The deletions are present in three different genetic backgrounds.
All open reading frames had to be deleted in the background of FY1679,
isogenic to S288C, the which was the source of the S. cerevisiae
genome sequencing. In addition to that for most genes an additional set
of deletions in either CEN.PK2
had been produced to prove that the deletion cassettes are of general use.
Strains constructed in phase I are identified by a 5 digit numerical code
in the accession number followed by terminal letter corresponding to the
mating type of the strain.
According to the guidelines of EUROFAN I all deletion
plasmids were also collected. In addition to that for most genes plasmids
for complementation of the deleted genes are available.
Worldwide gene deletion project (EUROFAN
In a worldwide effort european and overseas laboratories
are combined in the consortium of the Saccharomyces
genome deletion project (Winzeler et al.: Science 285 (1999)
901-906). The European task is labelled as EUROFAN II. All constructed
strains will be collected by EUROSCARF. The deletions are made in a genetic
background isogenic to S288c, the Saccharomyces cerevisiae strain
whose DNA sequence had been determined. Deletion strains constructed during
phase II were all in the genetic background of the BY
The EUROSCARF accession numbers of these strains differs
from the record number given by the Saccharomyces genome deletion
project only by the preceeding letter Y.