EUROpean Saccharomyces Cerevisiae

ARchive for Functional Analysis
 
Gene tagging and gene replacement using recombinase-mediated cassette
exchange in Schizosaccharomyces pombe

Adam T. Watson, Valerie Garcia, Neil Bone, Antony M. Carr and  John Armstrong 

Gene 407 (2008) 63-74

Plasmids for gene tagging and gene replacement using Cre recombinase-mediated cassette exchange (RMCE). The cassette consists of a S. pombe selectable marker flanked by a wild-type loxP site at one end and by a modified heterospecific lox site (loxM3) at the other. The cassette is stable because the flanking lox sites cannot recombine with each other. 

Please find additional information concerning ordering, shipment, and invoicing  under ordering conditions.

Base strain construction and cassette exchange:
accession no. plasmid name E.coli
transformation marker		  
P30537 pAW1 AmpR  
P30538 pAW5 AmpR  
P30539 pAW12 AmpR  
P30540 pAW31 AmpR  
P30541 pAW32 AmpR  
P30542 pAW33 AmpR  
P30543 pAW34 AmpR  
P30544 pAW41 AmpR  
P30545 pAW8 AmpR  
P30585 pAW8-XhoI AmpR new

Why pAW8-XhoI?


Different plasmids for C-terminal tagging:
accession no. plasmid name E.coli
transformation marker		  
P30546 pAW8-yEGFP AmpR  
P30547 pAW8-yECFP AmpR  
P30548 pAW8-yECitrine AmpR  
P30549 pAW8-13myc AmpR  
P30550 pAW8-3HA AmpR  
P30586 pAW8-1xFLAG AmpR new
P30587 pAW8-6xFLAG AmpR new
P30588 pAW8-6xHis AmpR new
P30589 pAW8-GST AmpR new
P30590 pAW8-mCherry AmpR new
P30591 pAW8-tdTomato AmpR new
P30592 pAW8-TAP AmpR new

  07-August-2009
MIPS 

SGD