EUROPEAN 
..........SACCHAROMYCES 
....................CEREVISIAE 
..............................ARCHIVE FOR 
........................................FUNCTIONAL ANALYSIS
EUROSCARF 
    Tetracycline dependent protein expression in Saccharomyces cerevisiae

    Three sets of plasmids for tetracycline dependent expression of gene products have been developed in the group of Enrique Herrero (Lleida, Spain). Development of tet plasmids has been continued in Manchester (Yen et al.). These plasmids are available from EUROSCARF. Please find a list of these plasmids below. The individual plasmids are still available. Please order by individual accession numbers.  You will be billed for each plasmid, as given in the handling fee list under ordering conditions.



accession no. plasmid marker reference
P30309 pCM148 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR) 1
P30310 pCM159 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of lacZ under control of tetO7 1
P30311 pCM161 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of lacZ under control of tetO2 1
P30312 pCM171 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of lacZ under control of tetO2 1
P30313 pCM172 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of lacZ under control of tetO2 1
P30314 pCM173 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of lacZ under control of tetO7 1
P30315 pCM174 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of lacZ under control of tetO7 1
P30316 pCM179 episomal yeast plasmid, marker URA3, tetracycline repressed expression of lacZ under control of tetO7 1
P30317 pCM180 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of target gene under control of tetO7 1
P30318 pCM181 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of target gene under control of tetO2 1
P30319 pCM182 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of target gene under control of tetO2 1
P30320 pCM183 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of target gene under control of tetO2 1
P30321 pCM184 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of target gene under control of tetO7 1
P30322 pCM185 centromeric yeast plasmid, marker TRP1, tetracycline repressed expression of target gene under control of tetO7 1
P30323 pCM186 centromeric yeast plasmid, marker URA3, tetracycline repressed expression of target gene under control of tetO7 1
P30324 pCM187 centromeric yeast plasmid, marker URA3, tetracycline repressed expression of target gene under control of tetO2 1
P30325 pCM188 centromeric yeast plasmid, marker URA3, tetracycline repressed expression of target gene under control of tetO2 1
P30326 pCM189 centromeric yeast plasmid, marker URA3, tetracycline repressed expression of target gene under control of tetO7 1
P30327 pCM190 episomal yeast plasmid, marker URA3, tetracycline repressed expression of target gene under control of tetO7 1
P30328 pCM217 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR)
 2
P30329 pCM218 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR')
 2
P30330 pCM223 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR')
 2
P30331 pCM240 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR-TUP1)
 2
P30332 pCM242 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR-SSN6)
 2
P30333 pCM243 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR'-TUP1)
 2
P30334 pCM244 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR'-SSN6)
 2
P30335 pCM245 plasmid for integrative yeast transformation, marker LEU2, adh1p(tetR'-SSN6)
 2
P30336 pCM246 plasmid for integrative yeast transformation, marker LEU2, adh1p(tetR'-TUP1)
 2
P30337 pCM247 plasmid for integrative yeast transformation, marker LEU2, adh1p(tetR-SSN6)
 2
P30338 pCM248 plasmid for integrative yeast transformation, marker LEU2, adh1p(tetR-TUP1)
 2
P30339 pCM251 centromeric yeast plasmid, marker TRP1, tetracycline inducible expression of target gene under control of tetO2
 2
P30340 pCM252 centromeric yeast plasmid, marker TRP1, tetracycline inducible expression of target gene under control of tetO7
 2
P30341 pCM224 promoter substitution cassette for one-step PCR integration of tetracycline repressable expression under control of tetO2, transformation marker KanMX
 3
P30342 pCM225 promoter substitution cassette for one-step PCR integration of tetracycline repressable expression under control of tetO7, transformation marker KanMX 3
P30343 pCM87 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR)
 1
P30344 pCM149 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR) 1
P30345 pCM150 plasmid for integrative yeast transformation, marker LEU2, CMVp(tetR) 1
P30383 pCM324 promoter substitution cassette for one-step PCR integration of tetracycline repressable expression under control of tetO2, transformation marker KanMX 4
P30384 pCM325 promoter substitution cassette for one-step PCR integration of tetracycline repressable expression under control of tetO7, transformation marker KanMX 4
P30385 pUG6-tTA tTA in pUG6
 4
Y40122 CML476 MATa ura3-52 leu2delta1 his3delta200 GAL2  CMVp(tetR’-SSN6)::LEU2 trp1::tTA 4


References:
1) Gari E, Piedrafita L, Aldea M, Herrero E. 
A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast. 1997 Jul; 13(9):837-48.
2) Belli G, Gari E, Piedrafita L, Aldea M, Herrero E. 
An activator/repressor dual system allows tight tetracycline-regulated gene expression in budding yeast. Nucleic Acids Res. 1998 Feb 15; 26(4):942-7.
3) Belli G, Gari E, Aldea M, Herrero E.
Functional analysis of yeast essential genes using a promoter-substitution cassette and the tetracycline-regulatable dual expression system. Yeast. 1998 Sep 15; 14(12):1127-38.
4) Yen K, Gitsham P, Wishart J, Oliver SG, Zhang N
An improved tetO promoter replacement system for regulating the expression of yeast genes. Yeast. 2003 (20): 1255-62.

 15-September-2011
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