EUROPEAN 
..........SACCHAROMYCES 
....................CEREVISIAE 
..............................ARCHIVE FOR 
........................................FUNCTIONAL ANALYSIS
EUROSCARF 
    Construction of a set of Saccharomyces cerevisiae vectors designed for recombinational cloning
    Vincent Van Mullem, Maxime Wery, Xavier De Bolle, and Jean Vandenhaute

    Yeast (2003) 20, 739 - 740

    The Gateway technology allows the transfer of any ORF flanked by specific recombination sites into any vectors harbouring the corresponding sites. These 20 Saccharomyces cerevisiae Gateway compatible vectors are designed for expression without tag sequence or for C- or N-terminal protein tagging with 3HA (haemagglutinin), 13myc, 4TAP (tandem affinity purification) or GST (glutathione S-transferase) epitopes. The centromeric vectors allow expression of DNA sequence in yeast under tetracycline-regulatable promoters, while expression from the high copy vectors is driven by PGK promoter. 

    accession no. plasmid name description
    P30205 pVV200 TRP1, 2 um
    P30206 pVV201 3HA, TRP1, 2 um
    P30207 pVV202 GST, TRP1, 2 um
    P30208 pVV203 13myc, TRP1, 2 um
    P30209 pVV204 TRP1, CEN
    P30210 pVV205 3HA, TRP1, CEN
    P30211 pVV206 GST, TRP1, CEN
    P30212 pVV207 13myc, TRP1, CEN
    P30213 pVV208 URA3, CEN
    P30214 pVV209 3HA, URA3, CEN
    P30215 pVV210 GST, URA3, CEN
    P30216 pVV211 13myc, URA3, CEN
    P30217 pVV214 URA3, 2 um
    P30218 pVV215 3HA, URA3, 2 um
    P30219 pVV216 GST, URA3, 2 um
    P30220 pVV217 13myc, URA3, 2 um
    P30221 pVV220 4TAP, TRP1, 2 um
    P30222 pVV221 4TAP, TRP1, CEN
    P30223 pVV222 4TAP, URA3, CEN
    P30224 pVV223 4TAP, URA3, 2 um

      8-Feb-2008
MIPS 

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