ARchive for Functional analysis
how to order
A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes.
Carsten Janke, Maria M. Magiera, Nicole Rathfelder, Christof Taxis, Simone Reber, Hiromi Maekawa, Alexandra Moreno-Borchart, Georg Doenges, Etienne Schwob, Elmar Schiebel, and Michael Knop. Yeast (2004) 21, 974 - 962
Transformed E.coli cells are resistant to ampicillin and kanamycin. The plasmid contains the kanMX gene from Tn903 that confers resistance to the aminoglycosid antibiotic G418 of transformed yeasts.
Plasmid usefull as template for PCR to create a C-terminal (1x) HA fusion protein of your gene of interest.
Download sequence as plain text file
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