EUROSCARF
EUROpean Saccharomyces Cerevisiae

ARchive for Functional analysis

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pYM27

Plasmid is part of the "PCR-TOOLBOX". For an individual plasmid order use accession number P30239.

Reference:
A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes.
Carsten Janke, Maria M. Magiera, Nicole Rathfelder, Christof Taxis, Simone Reber, Hiromi Maekawa, Alexandra Moreno-Borchart, Georg Doenges, Etienne Schwob, Elmar Schiebel, and Michael Knop. Yeast (2004) 21, 974 - 962


Transformed E.coli cells are resistant to ampicillin and kanamycin. The plasmid contains the kanMX gene from Tn903 that confers resistance to the aminoglycosid antibiotic G418 of transformed yeasts.
Plasmid usefull as template for PCR to create a C-terminal EGFP fusion protein of your gene of interest.


Plasmid Map pYM27


Download sequence as plain text file


 
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23-Jun-2005
Matthias Rose