ARchive for Functional analysis
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EUROpean
Saccharomyces
Cerevisiae
ARchive for Functional analysis |
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search yeast strains plasmids
how to order about EUROSCARF MIPS SGD |
pYM20 Reference: A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Carsten Janke, Maria M. Magiera, Nicole Rathfelder, Christof Taxis, Simone Reber, Hiromi Maekawa, Alexandra Moreno-Borchart, Georg Doenges, Etienne Schwob, Elmar Schiebel, and Michael Knop. Yeast (2004) 21, 974 - 962 Transformed E.coli cells are resistant to ampicillin. The plasmid contains the hph gene from Klebsiella pneumoniae encoding hygromycin B phosphotransferase and confers resistance to the antibiotic hygromycin B of transformed yeasts. Plasmid usefull as template for PCR to create C-terminal 9x myc tagged fusion proteins of your gene of interest. 
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