EUROSCARF
EUROpean Saccharomyces Cerevisiae

ARchive for Functional analysis

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pYM16

Plasmid is part of the "PCR-TOOLBOX". For an individual plasmid order use accession number P30302.

Reference:
A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes.
Carsten Janke, Maria M. Magiera, Nicole Rathfelder, Christof Taxis, Simone Reber, Hiromi Maekawa, Alexandra Moreno-Borchart, Georg Doenges, Etienne Schwob, Elmar Schiebel, and Michael Knop. Yeast (2004) 21, 974 - 962


Transformed E.coli cells are resistant to ampicillin. The plasmid contains the hph gene from Klebsiella pneumoniae encoding hygromycin B phosphotransferase and confers resistance to the antibiotic hygromycin B of transformed yeasts.
Plasmid usefull as template for PCR to create C-terminal 6xHA tagged fusion proteins of your gene of interest.


Plasmid Map pYM16


Download sequence as plain text file


 
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23-Jun-2005
Matthias Rose