• Chr I
• Chr II
• Chr III
• Chr IV
• Chr V
• Chr VI
• Chr VII
• Chr VIII
• Chr IX
• Chr X
• Chr XI
• Chr XII
• Chr XIII
• Chr XIV
• Chr XV
• Chr XVI
• Ref. strains

pVV223

Construction of a set of Saccharomyces cerevisiae vectors designed for recombinational cloning. Vincent Van Mullem, Maxime Wery, Xavier De Bolle, and Jean Vandenhaute. Yeast (2003) 20, 739 - 740

Transformed E.coli cells are resistant to ampicillin. The plasmid pVV223 contains the URA3 gene as transformation marker for selecting Saccharomyces cerevisiae transformants. The design of the applied PCR primers directs the PCR fragment to be fused behind a PGK promoter for the expression of a N-terminal 4TAP tagged fusion protein of your gene of interest. Expermental details are described in the above mentioned reference.





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