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    pUG6

Plasmid is part of the " DEL-MARKER-SET ". For an individual plasmid order use accession number P30114.

Reference:
Güldener, U., Heck, S., Fiedler, T., Beinhauer, J., and Hegemann, J.H. 1996: A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Research 24, 2519-2524
.

Transformed E.coli cells are resistant to ampicillin and kanamycin. The plasmid contains the kanMX gene from Tn903 that confers resistance to the aminoglycosid antibiotic G418 of transformed yeasts. Due to the flanking loxP sites the deletion marker can be removed by expressing cre recombinase.

Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants.


Plasmid Map pUG6


Download sequence as plain text file


 

14-Feb-2005
Matthias Rose

MIPS

SGD