pUG27

	EUROPEAN .......... S ACCHAROMYCES .................... CEREVISIAE .............................. ARCHIVE FOR ........................................ FUNCTIONAL ANALYSIS
EUROSCARF Home Description Address Collection index Chr I Chr II Chr III Chr IV Chr V Chr VI Chr VII Chr VIII Chr IX Chr X Chr XI Chr XII Chr XIII Chr XIV Chr XV Chr XVI Ref. strains Ordering conditions Order form Staff	pUG27 Plasmid is part of the " DEL-MARKER-SET ". For an individual plasmid order use accession number P30115. Reference: Güldener, U., Heinisch, J., Köhler G.J., Voss, D., and Hegemann, J.H. 2002: A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Research 30, e23 . Transformed E.coli cells are resistant to ampicillin. The plasmid contains the his5⁺ gene from Schizosaccharomyces pombe that complements the Saccharomyces cerevisiae his3 mutation. Due to the flanking loxP sites the deletion marker can be removed by expressing cre recombinase. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants. Download sequence as plain text file 14-Feb-2005 Matthias Rose
MIPS SGD