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    pSH47

Plasmid is part of the " DEL-MARKER-SET ". For an individual plasmid order use accession number P30119.

Reference:
Güldener, U., Heck, S., Fiedler, T., Beinhauer, J., and Hegemann, J.H. 1996: A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Research 24, 2519-2524
.

Transformed E.coli cells are resistant to ampicillin. The plasmid contains the cre recombinase gene under control of a GAL promoter and complements the ura3 mutation in transformed Saccharomyces cerevisiae strains.

The pSH47 plasmid is usefull to express the cre recombinase under Galactose control. loxP flanked deletion markers will be removed from yeast deletions.


Plasmid Map pSH47


Download sequence as plain text file


 

14-Feb-2005
Matthias Rose

MIPS

SGD