EUROSCARF
EUROpean Saccharomyces Cerevisiae

ARchive for Functional Analysis
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pKT128

 

overview of plasmids deposited by Sheff and Thorn

Accession number P30174
 

Reference:

Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae

Mark A. Sheff and Kurt S. Thorn Yeast 2004 21: 661 - 670


Tansformed E.coli cells are resistant to ampicillin.
Module for C-terminal tagging
The plasmid pKT128 contains the Schizosaccharomyces pombe his5 gene as transformation marker for selecting Saccharomyces cerevisiae transformants. Sphis5 complements the S. cerevisiae his3 mutation.



 


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Download map and sequence as pdf

27-May-2010
Peter Kötter