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pGREG600 Reference: Drag & drop Cloning in Yeast. Gregor Jansen, Cunle Wu, Babette Schade, David Y. Thomas, Malcolm Whiteway. Gene 2005 (344) 43 - 51 Transformed E.coli cells are resistant to ampicillin. The plasmid contains the URA3 gene as transformation marker for selecting yeast transformants. The design of the applied PCR primers directs the PCR fragment to form a C terminal GFP fusion protein of your gene of interest.
Download sequence as plain text file 20-Jul-2005
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