..........SACCHAROMYCES
....................CEREVISIAE
..............................ARCHIVE FOR
........................................FUNCTIONAL ANALYSIS
In vivo homologous recombination is used to introduce genes of interest into galactose-inducible expression vectors (pGREGs), permitting the formation of amino-terminal fusions. All vectors contain common regions for recombination that flank the stuffer fragment. Introduction of common recombination sequences at the end of PCR fragments will permit the cloning of genes without the need for specific restriction sites. In this process, the selectable stuffer HIS3 gene is replaced by successful gene integration, and a screen for loss of the selection marker identifies potential recombinants.
Due to low ordering frequency the "pGREG-SET" with a reduced handling fee will no longer be dispatched. The individual plasmids are still available. Please order by individual accession numbers. You will be billed for each plasmid, as given in the handling fee list under ordering conditions.
| accession no. | plasmid name | description |
| P30348 | pGREG503 | HIS3 |
| P30349 | pGREG504 | TRP1 |
| P30350 | pGREG505 | LEU2 |
| P30351 | pGREG506 | URA3 |
| P30352 | pGREG515 | N term 6x myc, LEU2 |
| P30353 | pGREG516 | N term 6x myc, URA3 |
| P30354 | pGREG523 | N term 13x myc, HIS3 |
| P30355 | pGREG524 | N term 13x myc, TRP1 |
| P30356 | pGREG525 | N term 13x myc, LEU2 |
| P30357 | pGREG526 | N term 13x myc, URA3 |
| P30358 | pGREG533 | N term 7x HA, HIS3 |
| P30359 | pGREG534 | N term 7x HA, TRP1 |
| P30360 | pGREG535 | N term 7x HA, LEU2 |
| P30361 | pGREG536 | N term 7x HA, URA3 |
| P30362 | pGREG543 | N term GST, HIS3 |
| P30363 | pGREG544 | N term GST, TRP1 |
| P30364 | pGREG545 | N term GST, LEU2 |
| P30365 | pGREG546 | N term GST, URA3 |
| P30366 | pGREG556 | N term myrGST, URA3 |
| P30367 | pGREG563 | N term caGFP, HIS3 |
| P30368 | pGREG564 | N term caGFP, TRP1 |
| P30369 | pGREG565 | N term caGFP, LEU2 |
| P30370 | pGREG566 | N term caGFP, URA3 |
| P30371 | pGREG573 | N term GFP, HIS3 |
| P30372 | pGREG574 | N term GFP, TRP1 |
| P30373 | pGREG575 | N term GFP, LEU2 |
| P30374 | pGREG576 | N term GFP, URA3 |
| P30375 | pGREG586 | N term 6x HIS, URA3 |
| P30376 | pGREG596 | N term myrGFP, URA3 |
| P30377 | pGREG597 | N term delta myrGFP, URA3 |
| P30378 | pGREG598 | N term caGFP, URA3 |
| P30379 | pGREG599 | C term caGFP, URA3 |
| P30380 | pGREG600 | C term GFP, URA3 |
| P30381 | pMDB | N term GAL4-BD, TRP1 |
| P30382 | pAD2 | N term GAL4-AD, LEU2 |