ID pCM251 XX AC XX DE Tetracycline-regulatable expression vector for Saccharomyces cerevisiae. XX KW tTA; TetO; tetracycline-regulatable promoter; expression vector; yeast. XX OS Expression vector. OC Artificial sequences. XX FH Key Location/Qualifiers FH FT source 1. .7407 FT /organism="Expression vector" FT CDS complement(28. .1035) FT /note="tTA reverse transactivator" FT /origin="pUHG17-1" FT misc_feature complement(1036. .1805) FT /note="CMV promoter" FT /origin="pUHG17-1" FT misc_feature 1817. .2028 FT /note="ADH1 transcriptional terminator" FT /origin="Saccharomyces cerevisiae" FT misc_feature 2034. .2133 FT /note="Two copies of the TetO box" FT /origin="Synthetic" FT misc_feature 2134. .2286 FT /note="CYC1 TATA region" FT /origin="pEMBLyex4" FT misc_feature 2289. .2342 FT /note="polylinker" FT misc_feature 2343. .2604 FT /note="CYC1 transcriptional terminator" FT /origin="Saccharomyces cerevisiae" FT misc_feature 2605. .7407 FT /note="YCplac22 centromeric vector FT with the TRP1 marker" XX pcm251.dat Length: 7407 February 27, 1998 12:39 Type: N Check: 2572 .. 1 GAATTCTTAT TACGATCCTC GCGCCCCcta CCCACCGTAC TCGTCAATTC 51 CAAGGGCATC GGTAAACATC TGCTCAAACT CGAAGTCGGC CATATCCAGA 101 GCGCCGTAGG GGGCGGAGTC GTGGGGGGTA AATCCCGGAC CCGGGGAATC 151 CCCGTCCCCC AACATGTCCA GATCGAAATC GTCTAGCGCG TCGGCATGCG 201 CCATCGCCAC GTCCTCGCCG TCTAAGTGGA GCTCGTCCCC CAGGCTGACA 251 TCGGTCGGGG GGGCCGTCGA CAGTCTGCGC GTGTGTCCCG CGGGGAGAAA 301 GGACAGGCGC GGAGCCGCCA GCCCCGCCTC TTCGGGGGCG TCGTCGTCCG 351 GGAGATCGAG CAGGCCCTCG ATGGTAGACC CGTAATTGTT TTTCGTACGC 401 GCGCGGCTGT ACGCGGACCC ACTTTCACAT TTAAGTTGTT TTTCTAATCC 451 GCATATGATC AATTCAAGGC CGAATAAGAA GGCTGGCTCT GCACCTTGGT 501 GATCAAATAA TTCGATAGCT TGTCGTAATA ATGGCGGCAT ACTATCAGTA 551 GTAGGTGTTT CCCTTTCTTC TTTAGCGACT TGATGCTCTT GATCTTCCAA 601 TACGCAACCT AAAGTAAAAT GCCCCACAGC GCTGAGTGCA TATAAcGCgT 651 TCTCTAGTGA AAAACCTTGT TGGCATAAAA AGGCTAATTG ATTTTCGAGA 701 GTTTCATACT GTTTTTCTGT AGGCCGTGTA tCTgAATGTA CTTTTGCTCC 751 ATtGCGATGA CTTAGTAAAG CACATCTAAA ACTTTTAGCG TTATTgCGTA 801 AAAAATCTTG CCAGCTTTCC CCTTtTAAAG GGCAAAAGTG AGTATGGTGC 851 CTATCTAACA TCTCAATGGC TAAGGCGTCG AGCAAAGCCC GCTTATTTTT 901 TACATGCCAA TACAgTGTAG GCTGCTCTAC ACCaAGCTTC TGGGCGAGTT 951 TACGGGTTGT TAAACCTTCG ATTCCGACCT CATTAAGCAG CTCTAATGCG 1001 CTGTTAATCA CTTTACTTTT ATCTAATCTA GAcatATGAA TTAATTCGGG 1051 CCGCGGAGGC TGGATCGGTC CCGGTGTCTT CTATGGAGGT CAAAACAGCG 1101 TGGATGGCGT CTCCAGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA 1151 TATAGACCTC CCACCGTACA CGCCTACCGC CCATTTGCGT CAATGGGGCG 1201 GAGTTGTTAC GACATTTTGG AAAGTCCCGT TGATTTTGGT GCCAAAACAA 1251 ACTCCCATTG ACGTCAATGG GGTGGAGACT TGGAAATCCC CGTGAGTCAA 1301 ACCGCTATCC ACGCCCATTG ATGTACTGCC AAAACCGCAT CACCATGGTA 1351 ATAGCGATGA CTAATACGTA GATGTACTGC CAAGTAGGAA AGTCCCATAA 1401 GGTCATGTAC TGGGCATAAT GCCAGGCGGG CCATTTACCG TCATTGACGT 1451 CAATAGGGGG CGTACTTGGC ATATGATACA CTTGATGTAC TGCCAAGTGG 1501 GCAGTTTAGC GTAAATACTC CACCCATTGA CGTCAATGGA AAGTCCCTAT 1551 TGGCGTTACT ATGGGAACAT ACGTCATTAT TGACGTCAAT GGGCGGGGGT 1601 CGTTGGGCGG TCAGCCAGGC GGGCCATTTA CCGTAAGTTA TGTAACGCGG 1651 AACTCCATAT ATGGGCTATG AACTAATGAC CCCGTAATTG ATTACTATTA 1701 ATAACTAGTC AATAATCAAT GTCAACATGG CGGTAATGTT GGACATGAGC 1751 CAATATAAAT GTACATATTA TGATATGGAT ACAACGTATG CAATGGGCCA 1801 AGCTCCTCGA GTAATTCGCG CCACTTCTAA ATAAGCGAAT TTCTTATGAT 1851 TTATGATTTT TATTATTAAA TAAGTTATAA AAAAAATAAG TGTATACAAA 1901 TTTTAAAGTG ACTCTTAGGT TTTAAAACGA AAATTCTTGT TCTTGAGTAA 1951 CTCTTTCCTG TAGGTCAGGT TGCTTTCTCA GGTATAGCAT GAGGTCGCTC 2001 TTATTGACCA CACCTCTACC GGCAGATCAA TTCCTCGATC CCTATCAGTG 2051 ATAGAGAGTC GACAAAGTCG AGTTTCTCGA TCCCTATCAG TGATAGAGAG 2101 TCGACAAAGT CGAGTTTCTC GATCGAGACC ACTGCATGCA TGTGCTCTGT 2151 ATGTATATAA AACTCTTGTT TTCTTCTTTT CTCTAAATAT TCTTTCCTTA 2201 TACATTAGGT CCTTTGTAGC ATAAATTACT ATACTTCTAT AGACACGCAA 2251 ACACAAATAC ACACACTAAA TTACCGGATC AATTCGGGGG ATCCGTTTAA 2301 ACAGGCCTGT TAACATCGAT AGCGGCCGCT AGGGCCCTGC AGGAGGGCCG 2351 CATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT 2401 CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT 2451 ATTTATTTTT TTATAGTTAT GTTAGTATTA AGAACGTTAT TTATATTTCA 2501 AATTTTTCTT TTTTTTCTGT ACAGACGCGT GTACGCATGT AACATTATAC 2551 TGAAAACCTT GCTTGAGAAG GTTTTGGGAC GCTCGAAGGC TTTAATTTGC 2601 GGCCAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT 2651 TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA 2701 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 2751 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA 2801 ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC 2851 TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 2901 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 2951 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 3001 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 3051 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 3101 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 3151 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 3201 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 3251 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 3301 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 3351 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 3401 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 3451 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 3501 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 3551 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 3601 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 3651 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 3701 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 3751 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 3801 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 3851 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 3901 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 3951 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 4001 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG 4051 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 4101 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 4151 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 4201 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 4251 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 4301 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC 4351 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 4401 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 4451 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 4501 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 4551 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 4601 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT 4651 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 4701 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 4751 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT 4801 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCAAG 4851 AATTAATTCG GTCGAAAAAA GAAAAGGAGA GGGCCAAGAG GGAGGGCATT 4901 GGTGACTATT GAGCACGTGA GTATACGTGA TTAAGCACAC AAAGGCAGCT 4951 TGGAGTATGT CTGTTATTAA TTTCACAGGT AGTTCTGGTC CATTGGTGAA 5001 AGTTTGCGGC TTGCAGAGCA CAGAGGCCGC AGAATGTGCA CTAGATTCCG 5051 ATGCTGACTT GCTGGGTATT ATATGTGTGC CCAATAGAAA GAGAACAATT 5101 GACCCGGTTA TTGCAAGGAA AATTTCAAGT CTTGTAAAAG CATATAAAAA 5151 TAGTTCAGGC ACTCCGAAAT ACTTGGTTGG CGTGTTTCGT AATCAACCTA 5201 AGGAGGATGT TTTGGCTCTG GTCAATGATT ACGGCATTGA TATCGTCCAA 5251 CTGCATGGAG ATGAGTCGTG GCAAGAATAC CAAGAGTTCC TCGGTTTGCC 5301 AGTTATTAAA AGACTCGTAT TTCCAAAAGA CTGCAACATA CTACTCAGTG 5351 CAGCTTCACA GAAACCTCAT TCGTTTATTC CCTTGTTTGA TTCAGAAGCA 5401 GGTGGGACAG GTGAACTTTT GGATTGGAAC TCGATTTCTG ACTGGGTTGG 5451 AAGGCAAGAG AGCCCCGAGA GCTTACATTT TATGTTAGCT GGTGGACTGA 5501 CGCCAGAAAA TGTTGGTGAT GCGCTTAGAT TAAATGGCGT TATTGGTGTT 5551 GATGTAAGCG GAGGTGTGGA GACAAATGGT GTAAAAGACT CTAACAAAAT 5601 AGCAAATTTC GTCAAAAATG CTAAGAAATA GGTTATTACT GAGTAGTATT 5651 TATTTAAGTA TTGTTTGTGC ACTTGCCTGC AAGCCTTTTG AAAAGCAAGC 5701 ATAAAAGATC TAAACATAAA ATCTGTAAAA TAACAAGATG TAAAGATAAT 5751 GCTAAATCAT TTGGCTTTTT GATTGATTGT ACAGGAAAAT ATACATCGCA 5801 GGGGGTTGAC TTTTACCATT TCACCGCAAT GGAATCAAAC TTGTTGAAGA 5851 GAATGTTCAC AGGCGCATAC GCTACAATGA CCCGATTCTT GCTAGCCTTT 5901 TCTCGGTCTT GCAAACAACC GCCGGCAGCT TAGTATATAA ATACACATGT 5951 ACATACCTCT CTCCGTATCC TCGTAATCAT TTTCTTGTAT TTATCGTCTT 6001 TTCGCTGTAA AAACTTTATC ACACTTATCT CAAATACACT TATTAACCGC 6051 TTTTACTATT ATCTTCTACG CTGACAGTAA TATCAAACAG TGACACATAT 6101 TAAACACAGT GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG 6151 TAAAGATTTC GTGTTCATGC AGATAGATAA CAATCTATAT GTTGATAATT 6201 AGCGTTGCCT CATCAATGCG AGATCCGTTT AACCGGACCC TAGTGCACTT 6251 ACCCCACGTT CGGTCCACTG TGTGCCGAAC ATGCTCCTTC ACTATTTTAA 6301 CATGTGGAAT TAATTCTCAT GTTTGACAGC TTATCATCGA ACTCTAAGAG 6351 GTGATACTTA TTTACTGTAA AACTGTGACG ATAAAACCGG AAGGAAGAAT 6401 AAGAAAACTC GAACTGATCT ATAATGCCTA TTTTCTGTAA AGAGTTTAAG 6451 CTATGAAAGC CTCGGCATTT TGGCCGCTCC TAGGTAGTGC TTTTTTTCCA 6501 AGGACAAAAC AGTTTCTTTT TCTTGAGCAG GTTTTATGTT TCGGTAATCA 6551 TAAACAATAA ATAAATTATT TCATTTATGT TTAAAAATAA AAAATAAAAA 6601 AGTATTTTAA ATTTTTAAAA AAGTTGATTA TAAGCATGTG ACCTTTTGCA 6651 AGCAATTAAA TTTTGCAATT TGTGATTTTA GGCAAAAGTT ACAATTTCTG 6701 GCTCGTGTAA TATATGTATG CTAAAGTGAA CTTTTACAAA GTCGATATGG 6751 ACTTAGTCAA AAGAAATTTT CTTAAAAATA TATAGCACTA GCCAATTTAG 6801 CACTTCTTTA TGAGATATAT TATAGACTTT ATTAAGCCAG ATTTGTGTAT 6851 TATATGTATT TACCCGGCGA ATCATGGACA TACATTCTGA AATAGGTAAT 6901 ATTCTCTATG GTGAGACAGC ATAGATAACC TAGGATACAA GTTAAAAGCT 6951 AGTACTGTTT TGCAGTAATT TTTTTCTTTT TTATAAGAAT GTTACCACCT 7001 AAATAAGTTA TAAAGTCAAT AGTTAAGTTT GATATTTGAT TGTAAAATAC 7051 CGTAATATAT TTGCATGATC AAAAGGCTCA ATGTTGACTA GCCAGCATGT 7101 CAACCACTAT ATTGATCACC GATATATGGA CTTCCACACC AACTAGTAAT 7151 ATGACAATAA ATTCAAGATA TTCTTCATGA GAATGGCCCA GCGATATATG 7201 CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG 7251 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG 7301 CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA 7351 TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC 7401 GGCCAGT