pAG61

	EUROPEAN .......... S ACCHAROMYCES .................... CEREVISIAE .............................. ARCHIVE FOR ........................................ FUNCTIONAL ANALYSIS
EUROSCARF Home Description Address Collection index Chr I Chr II Chr III Chr IV Chr V Chr VI Chr VII Chr VIII Chr IX Chr X Chr XI Chr XII Chr XIII Chr XIV Chr XV Chr XVI Ref. strains Ordering conditions Order form Staff	pAG61 Plasmid is part of the " DEL-MARKER-SET ". For an individual plasmid order use accession number P30112. Reference: Goldstein, A.L., Pan, X., and McCusker, J.H. 1999: Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae . Yeast 15, 507-511. Transformed E. coli cells are resistant to ampicillin. The plasmid contains the URA3 gene from Candida albicans and confers uracil prototrophy of transformed auxotrophic yeasts. In contrast to pAG60 this plasmid contains direct repeats of 500 bp from Aschbya gossypii flanking the insert. Plasmid usefull as template for PCR to create gene deletions. Integrative transformation of the respective PCR fragment and selection of the mutants. Provides the possiblity for a marker rescue by homologous recombination at the direct repeats. Counterselection with floro orotic acid. Download sequence as plain text file 14-Feb-2005 Matthias Rose
MIPS SGD