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Kötter P, Weigand JE, Meyer B, Entian K-D, Suess B

NAR (2009) 37: e120

A new artificial regulatory system for essential genes in yeast which prevents translation of target mRNAs upon tetracycline binding to aptamers introduced in the 5'UTRs. Exploiting direct RNA-ligand interaction renders auxiliary protein factors unnecessary. Therefore, the method is strain independent and not susceptible to interferences by heterologous expressed regulatory proteins . 

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