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  One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins    Denis Lafontaine and David Tollervey
Nucleic Acids Research (1996) 24, 3469-3472  
    A technique which allows the initial characterization of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR.
   

accession no.	strain / plasmid name	description
Y40021	YDL401	MATa; ura3-52; (trp1-D63 or trp1-289); leu2-D1; his3-D200; gal2; galD108
Y40022	YDL402	MATalpha; ura3-52; (trp1-D63 or trp1-289); leu2-D1; his3-D200; gal2; galD108
P30199	pTL26	basis of pTL27, pTL28, and pTL32
P30200	pTL27	plasmid for N-terminal ProtA tag
P30201	pTL28	plasmid for N-terminal c-myc tag
P30202	pTL32	plasmid for N-terminal His8 tag

 

  08-Oct-2003
Matthias Rose

SGD