EUROpean Saccharomyces Cerevisiae

ARchive for Functional Analysis
 

New Plasmids from the EMBL


We have received 103 plasmids from the group of Jan Ellenberg. This web page will be updated in short time intervals.

The use of the plasmids listed on this page is restricted to academic users for pure academic research. Before dispatching the plasmids we need a signed material transfer agreement. Please download the material transfer agreement, and send two signed copies to EUROSCARF. Upon request we will return one countersigned material transfer agreement together with the plasmids.

Please find additional information concerning ordering, shipment, and invoicing  under ordering conditions .

 

Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis
Jan Ellenberg, Eric D. Siggia, Jorge E. Moreira, Carolyn L. Smith, John F. Presley, Howard J. Worman, and Jennifer Lippincott-Schwartz
J Cell Biol (1997) 138, 1193-1206
accession no. plasmid name E.coli transformation marker
P30453 pLBR1TM-GFP KanR

Dual-colour imaging with GFP variants
Jan Ellenberg, Jennifer Lippincott-Schwartz and John F. Presley
Trends Cell Biol (1999) 9, 52-56
accession no. plasmid name E.coli transformation marker
P30454 pLBR1TM-YFP KanR

Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells
Nathalie Daigle, Joël Beaudouin, Lisa Hartnell, Gabriela Imreh, Einar Hallberg, Jennifer Lippincott-Schwartz and Jan Ellenberg
J Cell Biol (2001) 154, 71-84
accession no. plasmid name E.coli transformation marker
P30455 pEGFP-LaminB1 KanR
P30456 pCFP-LaminB1 KanR
P30457 pEGFP-Nup153 KanR
P30458 pEGFP3-NupP153 KanR
P30459 pPOM121-EGFP3 KanR
P30460 pSRb-ECFP KanR
P30650 pNup153-EGFP KanR          new

Four-dimensional imaging and quantitative reconstruction to analyse complex spatiotemporal processes in live cells
Daniel Gerlich, Joël Beaudouin, Matthias Gebhard, Jan Ellenberg and Roland Eils
Nat Cell Biol (2001) 3, 852-855
accession no. plasmid name E.coli transformation marker
P30461 pgammaTubulin-DsRed1 KanR

Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina
Joël Beaudouin, Daniel Gerlich, Nathalie Daigle, Roland Eils and Jan Ellenberg
Cell (2002) 108, 83-96
accession no. plasmid name E.coli transformation marker
P30462 pYFP-LaminB1 KanR
P30463 pEGFP-LAP2b KanR
P30464 pYFP-LAP2b KanR
P30465 pYFP-alphaTubulin KanR

Global chromosome positions are transmitted through mitosis in mammalian cells
Daniel Gerlich, Joël Beaudouin, Bernd Kalbfuss, Nathalie Daigle, Roland Eils and Jan Ellenberg
Cell (2003) 112, 751-764
accession no. plasmid name E.coli transformation marker
P30466 pH2B-DiHcRed1 KanR
P30467 pH2B-YFP KanR
P30468 pEGFP-CENPA KanR

Nuclear envelope breakdown in starfish oocytes proceeds by partial NPC disassembly followed by a rapidly spreading fenestration of nuclear membranes
Péter Lénárt, Gwénaël Rabut, Nathalie Daigle, Arthur R. Hand, Mark Terasaki and Jan Ellenberg
J Cell Biol (2003) 160, 1055-1068
accession no. plasmid name E.coli transformation marker
P30469 pGEMHE-EGFP-LaminB1 AmpR
P30470 pGEMHE-EGFP-Nup98 AmpR
P30471 pGEMHE-EGFP-Nup153 AmpR
P30472 pNup214-EGFP KanR
P30473 pGEMHE-EGFP-Nup214 AmpR
P30474 pGEMHE-POM121-EGFP3 AmpR

Mapping the dynamic organization of the nuclear pore complex inside single living cells
Gwénaël Rabut, Valérie Doye and Jan Ellenberg
Nat Cell Biol (2004) 6, 1114-1121
accession no. plasmid name E.coli transformation marker
P30475 pEGFP-hALADIN KanR
P30476 pCG1-EGFP3 KanR
P30477 pGp210-EGFP3 KanR
P30478 pEGFP-Importin B1 KanR
P30479 pEGFP-Nup35 KanR
P30480 pEGFP-Nup37 KanR
P30481 pEGFP-Nup43 KanR
P30482 pEGFP2-Nup50 KanR
P30483 pEGFP2-Nup58 KanR
P30484 pNup62-EGFP3 KanR
P30485 pEGFP3-Nup85 KanR
P30486 pNup93-EGFP3 KanR
P30487 pEGFP-Nup98 KanR
P30488 pEGFP-Nup214 KanR
P30489 pSec13-EGFP3 KanR
P30490 pEGFP3-Seh1 KanR

A contractile nuclear actin network drives chromosome congression in oocytes
Péter Lénárt, Christian P. Bacher, Nathalie Daigle, Arthur R. Hand, Roland Eils, Mark Terasaki and Jan Ellenberg
Nature (2005) 436, 812-818
accession no. plasmid name E.coli transformation marker
P30491 pGEMHE-EGFP–ABD AmpR
P30492 pGEMHE-ensconsin–EGFP3 AmpR
P30493 pGEMHE-H2B-DiHcRed1 AmpR

Dissecting the contribution of diffusion and interactions to the mobility of nuclear proteins
Joël Beaudouin, Felipe Mora-Bermúdez, Thorsten Klee, Nathalie Daigle and Jan Ellenberg
Biophys J (2006) 90, 1878-1894
accession no. plasmid name E.coli transformation marker
P30494 pbeta-gal-DiHcRed1 KanR
P30495 pH1.2-PAGFP KanR
P30496 pH1.3-PAGFP KanR
P30497 pH1.4-PAGFP KanR
P30498 pH1.5-PAGFP KanR
P30499 pH2B-PAGFP KanR
P30500 pEGFP-HP1b KanR
P30501 pEGFP-RCC1 KanR
P30502 pPAGFP-RCC1 KanR
P30503 pH1.1-PAGFP KanR

Condensin I stabilizes chromosomes mechanically through a dynamic interaction in live cells
Daniel Gerlich, Toru Hirota, Birgit Koch, Jan-Michael Peters and Jan Ellenberg
Curr Biol (2006) 16, 333-344
accession no. plasmid name E.coli transformation marker
P30506 pEGFP-Kleisin-gamma KanR
P30507 pEGFP-Kleisin-beta KanR

Live-cell imaging reveals a stable Cohesin-Chromatin interaction after but not before DNA replication
Daniel Gerlich, Birgit Koch, Florine Dupeux, Jan-Michael Peters and Jan Ellenberg
Curr Biol (2006) 16, 1571-1578
accession no. plasmid name E.coli transformation marker
P30504 pEGFP-Scc1 KanR
P30505 pEGFP-SA1 KanR

Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum
Keppler A, Arrivoli C, Sironi L, Ellenberg J
Biotechniques (2006) 41, 167-175
accession no. plasmid name E.coli transformation marker
P30508 pH2B-mRFP KanR
P30509 pMAGT-H2B KanR

λN-GFP: an RNA reporter system for live-cell imaging
Nathalie Daigle and Jan Ellenberg
Nat Methods (2007) 4, 633-636
accession no. plasmid name E.coli transformation marker
P30510 p4LambdaN22-3mEGFP-M9 KanR
P30511 pmRFP-4boxB-beta-actin-zipcode KanR
P30512 prprb-mRFP-4boxB KanR
P30513 p5boxB-SRPRNA KanR
P30514 pSRP19-mRFP KanR
P30551 prprb-mRFP-16boxB KanR
P30552 pmRFP-16boxB-beta-actin-zipcode KanR

Maximal chromosome compaction occurs by axial shortening in anaphase and depends on Aurora kinase
Felipe Mora-Bermudez, Daniel Gehrlich and Jan Ellenberg
Nat Cell Biol (2007) 9, 822-841
accession no. plasmid name E.coli transformation marker
P30515 pEGFP-H2B KanR
P30516 pmEGFP-alpha-tubulin KanR

Self-organization of MTOCs replaces centrosome function during acentrosomal spindle assembly in live mouse oocytes
Melina Schuh and Jan Ellenberg
Cell (2007) 130, 484-498
accession no. plasmid name E.coli transformation marker
P30517 pGEMHE-H2B-mRFP AmpR
P30518 pGEMHE-EGFP-MAP4 AmpR
P30519 pGEMHE-EB3-mEGFP AmpR
P30520 pGEMHE-C-moesin-mRFP AmpR

Systematic kinetic analysis of mitotic dis- and reassembly of the nuclear pore in living cells
Dultz E, Zanin E, Wurzenberger C, Braun M, Rabut G,  Sironi L and  Ellenberg J
J Cell Biol (2008) 180, 857-865
accession no. plasmid name E.coli transformation marker
P30553 pIBB-DiHcRed KanR
P30554 pPOM121-mCherry KanR
P30606 pLBR1TM-mCherry KanR

A new model for asymmetric spindle positioning in mouse oocytes
Schuh M and Ellenberg J
Curr Biol (2008) 18, 1986-1992
accession no. plasmid name E.coli transformation marker
P30607 pCS2-Fmn2-mCherry AmpR

Chromophore-Assisted Laser Inactivation of alpha- and gamma-Tubulin SNAP-tag Fusion Proteins inside Living Cells
Keppler A and Ellenberg J
ACS Chem Biol (2009) 4(2), 127-138
accession no. plasmid name E.coli transformation marker
P30608 pGamma-tubulin-SNAP-tag KanR
P30609 pEB3-mCherry KanR
P30610 pmCherry-MAP4 KanR


 

Structure and nuclear import function of the C-terminal domain of influenza virus polymease PB2 subunit

Tarendeau F, Boudet J, Guilligay D, Mas PJ, Bougault CM, Boulo S, Baudin F, Ruigrok RW, Daigle N, Ellenberg J, Cusack S, Simorre JP and Hart DJ

Nat Struct Mol Biol 2007 14(3): 229 - 233

accession no. plasmid name E.coli transformation marker
P30613 pmEGFP-PB2 KanR
P30614 pmEGFP-PB2_K738Q_K752N_R755Q KanR

 

Nuclear import and assembly of the influenza A virus RNA polymerase studied in live cells by Fluorescence Cross Correlation Spectroscopy

Huet S, Avilov S, Ferbitz L, Daigle N, Cusack S and Ellenberg J

J Virol (2010) 84(3), 1254-1264

accession no. plasmid name E.coli transformation marker
P30615 pPB1-mEGFP KanR
P30616 pPA-mCherry KanR
P30617 pPB1-mCherry KanR
P30618 pmCherry-PB2 KanR
P30619 pPA-mCFP KanR
P30620 pPB1-mCFP KanR
P30621 pmCFP-PB2 KanR
P30622 pmEGFP-MBP-mCherry KanR

Molecular crowding affects diffusion and binding of nuclear proteins in heterochromatin and reveals the fractal organization of chromatin

Bancaud A, Huet S, Daigle N, Mozziconacci J, Beaudouin J and Ellenberg J

EMBO J (2009) 28(24) 3785-98

accession no. plasmid name E.coli transformation marker
P30623 pmEGFP2 KanR
P30624 pmEGFP5 KanR
P30625 pmEGFP10 KanR
P30626 pmRFP2 KanR
P30627 pmPAGFP2 KanR
P30628 pH1t-mPAGFP KanR

Monitoring the permeability of the nuclear envelope during the cell cycle

Lénárt P and Ellenberg J

Methods (2006) 38(1) 17-24

accession no. plasmid name E.coli transformation marker
P30629 pMBP-EGFP KanR
P30630 pEGFP-M9 KanR
P30631 pIBB-EGFP KanR

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

Neumann B, Walter T, Hériché JK, Bulkescher J, Erfle H, Conrad C, Rogers P, Poser I, Held M, Liebel U, Cetin C, Sieckmann F, Pau G, Kabbe R, Wünsche A, Satagopam V, Schmitz MH, Chapuis C, Gerlich DW, Schneider R, Eils R, Huber W, Peters JM, Hyman AA, Durbin R, Pepperkok R and Ellenberg J.

Nature (2010) 464 721-727

accession no. plasmid name E.coli transformation marker
P30632 pH2B-mCherry KanR


 

Live imaging of single nuclear pores reveals unique assembly kinetics and mechanism in interphase

Dultz E and Ellenberg J

J Cell Biol (2010) 191 15 - 22

accession no. plasmid name E.coli transformation marker
P30648 pPOM121-3mCherry KanR
P30649 p3mCherry-Nup107 KanR


 

Intracellular transport by an anchored homogeneously contracting F-actin meshwork

Mori M, Monnier N, Daigle N, Bathe M, Ellenberg J and Lénárt P.

Curr Biol (2011) 21 606-611

accession no. plasmid name E.coli transformation marker
P30651 pMS2-H2B-EGFP AmpR
P30652 pGEMHE-H2B-mCherry AmpR
P30653 pGEMHE-3mEGFP-UtrCH AmpR

 

Complete kinetochore tracking reveals error-prone homologous chromosome biorientation in mammalian oocytes

Kitajima TS, Ohsugi M, Ellenberg J.

Cell (2011)146 568-581

accession no. plasmid name E.coli transformation marker
P30659 pGEMHE-EGFP-CEN-P1 AmpR
P30660 pGEMHE-3mCherry-CENP-P1 AmpR
P30661 pGEMHE-Mad2-mEGFP AmpR


The use of the plasmids listed on this page is restricted to academic users for pure academic research. Before dispatching the plasmids we need a signed material transfer agreement. Please download the material transfer agreement, and send two signed copies to EUROSCARF. Upon request we will return one countersigned material transfer agreement together with the plasmids.

Please find additional information concerning ordering, shipment, and invoicing  under ordering conditions .

 
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