ARchive for Functional Analysis
DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae
Mara K. Voracheck-Warren and John H. McCusker
new dominant resistance marker, dsdAMX4, for use in the genetic
manipulation of S. cerevisiae. The dsdA gene, which is
derived from Escherichia coli and encodes a D-serine deaminase,
confers to S. cerevisiae resistance to D-serine and the ability to
use D-serine as a nitrogen source.
dsdAMX4 cassette is a
new marker for use in chromosomal gene disruption. The unique selection
properties of the dsdAMX4 cassette make it an important addition to the
existing array of S. cerevisiae genetic tools.
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