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Ivan Sadowski, Ting-Cheng Su and Jennifer Parent

Yeast 2007 24: 447 - 455

Vectors were developed for two-step chromosomal integration of reporter genes or expression constructs.  Targets of integration are the ADE8, LYS2, MET15, LEU2, HIS3 or FCY1 genes. Integration using these "disintegrator" vectors produces a single-copy integration of the construct of interest at the junction of the marker deletion, and removes the additional plasmid sequences. The integrated constructs do not contain flanking sequence duplications, and therefore should be highly stable.

Please find additional information concerning ordering, shipment, and invoicing  under ordering conditions.

Due to discrepancies found by restriction analysis plasmid pIS373 was partially re-sequenced.
[EUROSCARF, Peter Kötter, 14-03-2011]

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