ARchive for Functional Analysis
Kit for making CUP1 t.s. degrons by PCR
Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo
Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo
Kanamaki, M., Sanchez-Diaz, A., Gambus, A. and Labib, K.
Nature 2003 423: 720 - 724
Rapid depletion of budding yeast protein by fusion to a heat-inducible degron
Rapid depletion of budding yeast protein by fusion to a heat-inducible degron
Sanchez-Diaz, A., Kanamaki, M., Marchesi, V. and Labib, K.
Please order the yeast strains or individual plasmids for individual handling fees. Due to low ordering
frequency the "DEGRON-KIT" with a reduced handling fee will no longer be dispatched.
Please find additional information concerning ordering, shipment, and invoicing under ordering conditions.
| accession no. | strain name | description |
| Y40020 | YKL200 | MATa; ade2-1; ura3-1; his3-11,15; trp1-1; leu2-3,112; can1-100; ubr1::GAL1p-HA-UBR1 (HIS3) |
| Y40121 | YMK165 | MATa/alpha; ade2-1/ade2-1; ura3-1/ura3-1; his3-11,15/his3-11,15; trp1-1/trp1-1; leu2-3,112/leu2-3,112; can1-100/can1-100; UBR1/ubr1::GAL1p-HA-UBR1 (HIS3) |
| accession no. | plasmid name | description |
| P30172 | pKL142 | vector for making GAL1p regulated UBR1 yeast strains |
| P30173 | pKL187 | vector for making CUP1 t.s. degrons by PCR |
Working instructions kindly provided by Karim Labib.
17-March-2011