ARchive for Functional Analysis
An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
Tilmann Bürckstümmer, Keiryn L Bennett, Adrijana Preradovic, Gregor Schütze, Oliver Hantschel, Giulio Superti-Furga and Angela Bauch
Although TAP was instrumental in elucidating the yeast cellular machinery, in mammalian cells the method suffers from a low overall yield. These dual-affinity tags were optimized for use in mammalian cells. A tag based on protein G and the streptavidin-binding peptide (GS-TAP) resulted in a tenfold increase in protein-complex yield and improved the specificity of the procedure. This allows purification of protein complexes that were hitherto not amenable to TAP and use of less starting material, leading to higher success rates and enabling systematic interaction proteomics projects.
The use of the plasmids listed on this page is restricted to academic users for pure academic research. Before dispatching the plasmids we need a signed material transfer agreement. Please download the material transfer agreement, and send two signed copies to EUROSCARF. Upon request we will return one countersigned material transfer agreement together with the plasmids.
Please find additional information concerning ordering, shipment, and invoicing under ordering conditions.
The plasmids can only be propagated in E. coli strains that tolerate the presence of the ccdB gene (e.g. DB3.1).