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Yen K, Gitsham P, Wishart J, Oliver SG, Zhang N

Yeast (2003) 20: 1255-1262

The authors have created a new, shorter, KanMX4-tetO cassette by removing the transactivator (tTA) sequence from the original cassette. The transactivator (tTA) has been integrated into the yeast genome to create a new strain for use with the new system, which has a greatly increased efficiency of promoter substitution.

Due to discrepancies found by restriction analysis plasmids pCM324 and pCM325 were partially re-sequenced.
[EUROSCARF, Peter Kötter, 25-01-2011]

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