ARchive for Functional Analysis
An improved tetO promoter replacement system for regulating the expression of yeast genes
Yen K, Gitsham P, Wishart J, Oliver SG, Zhang N
authors have created a new, shorter, KanMX4-tetO cassette by removing
the transactivator (tTA) sequence from the original cassette. The transactivator (tTA) has been integrated into the yeast genome to
create a new strain for use with the new system, which has a greatly increased efficiency of promoter substitution.
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