LOCUS pFA6a-VN-HIS3MX6 4594 bp DNA CIRCULAR SYN 19-JAN-2010 DEFINITION ACCESSION pFA6a-VN-HIS3MX6 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4594) AUTHORS Self JOURNAL Unpublished. COMMENT Due to discrepancies found by restriction analysis plasmids containing the HIS3MX6 marker cassette were partially re- sequenced. By this it was obviously that the origin of the HIS3 was not Schizosaccharomyces pombe but Saccharomyces klyuveri. [EUROSCARF, Peter Kötter, 19-01-2010] FEATURES Location/Qualifiers CDS 78..599 /gene="VN" /product="N-terminal fragment of Venus" misc_feature 607..809 /gene="ADH1t" /product="ADH1 terminator" misc_feature 847..1225 /gene="TEFp" /product="TEF1 promoter of A. gossypii" CDS 1226..1924 /gene="SkHIS3" /product="Saccharomyces klyuveri HIS3" misc_feature 1927..2159 /gene="TEF1t" /product="TEF1 terminator of A. gossypii" CDS complement (3267..4127) /gene="AmpR" BASE COUNT 1190 a 1136 c 1104 g 1164 t ORIGIN 1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta 61 acagatccat cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca 121 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg 181 agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc 241 ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct 301 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc 361 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt 421 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg 481 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg 541 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgagtagg 601 gcgcgccact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt 661 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt 721 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agtatgaggt 781 cgctcttatt gaccacacct ctaccggcag atccgctagg gataacaggg taatatagat 841 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata 901 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca 961 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg 1021 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca 1081 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt 1141 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc 1201 acatcacatc cgaacataaa caaccatggc agaaccagcc caaaaaaagc aaaaacaaac 1261 tgttcaggag cgcaaggcgt ttatctcccg tatcactaat gaaactaaaa ttcaaatcgc 1321 tatttcgctg aatggtggtt atattcaaat aaaagattcg attcttcctg caaagaagga 1381 tgacgatgta gcttcccaag ctactcagtc acaggtcatc gatattcaca caggtgttgg 1441 ctttttggat catatgatcc atgcgttggc aaaacactct ggttggtctc ttattgttga 1501 atgtattggt gacctgcaca ttgacgatca ccatactacc gaagattgcg gtatcgcatt 1561 agggcaagcg ttcaaagaag caatgggtgc tgtccgtggt gtaaaaagat tcggtactgg 1621 gttcgcacca ttggatgagg cgctatcacg tgccgtagtc gatttatcta gtagaccatt 1681 tgctgtaatc gaccttggat tgaagagaga gatgattggt gatttatcca ctgaaatgat 1741 tccacacttt ttggaaagtt tcgcggaggc ggccagaatt actttgcatg ttgattgtct 1801 gagaggtttc aacgatcacc acagaagtga gagtgcgttc aaggctttgg ctgttgccat 1861 aagagaagct atttctagca atggcaccaa tgacgttccc tcaaccaaag gtgttttgat 1921 gtgaagagta ctgacaataa aaagattctt gttttcaaga acttgtcatt tgtatagttt 1981 ttttatattg tagttgttct attttaatca aatgttagcg tgatttatat tttttttcgc 2041 ctcgacatca tctgcccaga tgcgaagtta agtgcgcaga aagtaatatc atgcgtcaat 2101 cgtatgtgaa tgctggtcgc tatactgctg tcgattcgat actaacgccg ccatccagtt 2161 taaacgagct cgaattcatc gatgatatca gatccactag tggcctatgc ggccgcggat 2221 ctgccggtct ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa 2281 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 2341 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 2401 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 2461 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 2521 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 2581 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 2641 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 2701 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 2761 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 2821 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 2881 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 2941 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 3001 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 3061 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 3121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 3181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 3241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 3301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 3361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 3421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 3481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 3541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 3601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 3661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 3721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 3781 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 3841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 3901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 3961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 4021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 4081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 4141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 4201 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 4261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 4321 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 4381 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 4441 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg 4501 agagtgcacc atatggacat attgtcgtta gaacgcggct acaattaata cataacctta 4561 tgtatcatac acatacgatt taggtgacac tata //