LOCUS pIS112 4725 bp DNA CIRCULAR SYN 14-MAR-2011 DEFINITION Integration vector pIS112, complete sequence. ACCESSION pIS112 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4725) AUTHORS Self JOURNAL Unpublished. COMMENT GenBank 4725 bp DNA circular SYN 09-APR-2007 ACCESSION EF173309 VERSION EF173309.1 GI:140047349 KEYWORDS: . Disintegrator vectors for single-copy yeast chromosomal integration Ivan Sadowski, Ting-Cheng Su and Jennifer Parent Yeast (2007) 24: 447 - 455 FEATURES Location/Qualifiers misc_feature 86..407 /gene="ade8 YIP-in" /product="3' flanking ade8 YIP-in fragment (+511,+805 relative to the start ATG)" CDS complement (807..1610) /gene="URA3" /product="URA3 encoding orotidine-5'-phosphate (OMP) decarboxylase" CDS 2029..2889 /gene="AmpR" /product="bla, encodingbeta-lactamase" misc_feature 3592..3664 /gene="ori" /product="origin of replication" misc_feature 3888..4725 /gene="ade8 YIP-out" /product="5' flanking ade8 YIP-out fragment (-847,-19 relative to the start ATG)" BASE COUNT 1276 a 1108 c 1042 g 1299 t ORIGIN 1 gcggccgcct cgagagatct aagcttatcg atgcatgcct gcaggtcgac tctagaggat 61 ccccggggta ccgagctcga attcagccat tggtggtaaa aaagctagaa atcatacctg 121 gcgaagaaac actagagcag tacgagcaaa gggtacacga tgctgagcac atcgctcctc 181 ggcgcgccga ggagcgattg tagaagctac atataaggtt ttacagcagc ttcacaaata 241 agaatagttc ccatatttat acttttatat ttggcattct tcaaagtgag gcgcgaaaca 301 tctcgcgtcg taaatcatga tcatggattg tgacaaaacg atcttaaagg tttcgaataa 361 aatgtgtgta aatacgtata cttatatatg aatgtgcatg tctcgacgcc tggcgtaata 421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc 481 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca 541 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac 601 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga 661 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac 721 gaaagggcgg gtaataactg atataattaa attgaagctc taatttgtga gtttagtata 781 catgcattta cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct 841 tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat 901 agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta 961 tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac 1021 caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa 1081 tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag 1141 cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct 1201 gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg 1261 tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca 1321 atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt 1381 agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt 1441 aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca 1501 tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca 1561 acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt 1621 cgtttcctgc atgtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt 1681 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct 1741 tccttctgtt cggagattac cgaatcaaaa aaatttcaaa gaaaccgaaa tcaaaaaaaa 1801 gaataaaaaa aaaatgatga attgaattga aaagctggcc tcgtgatacg cctattttta 1861 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 1921 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 1981 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 2041 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 2101 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 2161 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 2221 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 2281 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 2341 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 2401 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 2461 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 2521 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 2581 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 2641 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 2701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 2761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 2821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 2881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 2941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 3001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 3181 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 3421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 3721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 3781 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 3841 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcagat cctaaacagg 3901 caaaggttca ttctttctga tccgttctat cagaaattta acggcttgaa ctctataagg 3961 atcagacttg cctactacta aagcgttctg caagaaattc ctgataaggt cctgtagaac 4021 ggccttgcga ccggaaaccg ggaaagatac agacctgcaa atatccttca attcggacac 4081 tttcagcagt tccatcagag tgatggtttc ctccacctcg tttcttagtt cattagtcgg 4141 ttccctatca ggcccaggcg tttcatcttc ccagtaatcc tctaaattta tcattgaaac 4201 agaatgtata tggaacactc aactgtttga gttggagtct tcttttcttg tgaaatatgg 4261 atgaactcca cttcattctt tcatattcat attaaagatg atacactttt caacttacta 4321 tctggtgcgg tgaatgggtg tatacattga ctacagctca ggtgacatct aggggccgtt 4381 tagtgtaaga atataaagta caatccaaaa aggaaaatta tacttgttta agaaaagagg 4441 aaccttctgc tcgctctaaa atggtagttc tagtgatagt ttaaaaatgc attttccttc 4501 cgacttcgaa gatagatttc agcataaaga ctaaaagcgt cagcaaggat tcgaatgagt 4561 gactcgttgc tagtgactct gacctgtttt ttattccagc aagaggaaag ttatatagag 4621 tagtttatga agagaagaca cttctaaagt tgaataacgc tcctagcctc aaggttgatg 4681 atttaacttg cagcaagcgc aggtgagagc caacacacat gaatt //