LOCUS pKT240 5331 bp DNA CIRCULAR SYN 24-MAR-2010 DEFINITION ACCESSION pKT240 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5331) AUTHORS Self JOURNAL Unpublished. COMMENT SECNOTES|Mark A. Sheff and Kurt S. Thorn. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast 21 (2004) 661-670 FEATURES Location/Qualifiers misc_feature 10..388 /gene="TEFp" /product="Ashbya gossypii TEF promoter" CDS 389..1087 /gene="SpHIS5" /product="Schizosaccharomyces pombe his5, imidazoleglycerol phosphate dehydratase" misc_feature 1094..1318 /gene="TEFt" /product="Ashbya gossypii TEF terminator" misc_feature 1653..1738 /gene="ori" /product="origin of replication" CDS complement (2428..3288) /gene="AmpR" /product="bla, beta-lactamase" misc_feature 3800..3826 /gene="linker" /product="optimized linker" CDS 3827..4540 /gene="yECitrine" /product="derived from yEYFP (Q69M)" misc_feature 4559..5056 /gene="13xmyc" /product="13xmyc tag" misc_feature 5101..5303 /gene="ADH1t" /product="ADH1 terminator" BASE COUNT 1516 a 1165 c 1219 g 1431 t ORIGIN 1 gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga 61 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt 121 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca 181 cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc 241 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg 301 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt 361 ctcacatcac atccgaacat aaacaaccat ggcagaacca gcccaaaaaa agcaaaaaca 421 aactgttcag gagcgcaagg cgtttatctc ccgtatcact aatgaaacta aaattcaaat 481 cgctatttcg ctgaatggtg gttatattca aataaaagat tcgattcttc ctgcaaagaa 541 ggatgacgat gtagcttccc aagctactca gtcacaggtc atcgatattc acacaggtgt 601 tggctttttg gatcatatga tccatgcgtt ggcaaaacac tctggttggt ctcttattgt 661 tgaatgtatc ggtgacctgc acattgacga tcaccatact accgaagatt gcggtatcgc 721 attagggcaa gcgttcaaag aagcaatggg tgctgtccgt ggtgtaaaaa gattcggtac 781 tgggttcgca ccattggatg aggcgctatc acgtgccgta gtcgatttat ctaatagacc 841 atttgctgta atcgaccttg gattgaagag agagatgatt ggtgatttat ccactgaaat 901 gattccacac tttttggaaa gtttcgcgga ggcggccaga attactttgc atgttgattg 961 tctgagaggt ttcaacgatc accacagaag tgagagtgcg ttcaaggctt tggctgttgc 1021 cataagagaa gctatttcta gcaatggcac caatgacgtt ccctcaacca aaggtgtttt 1081 gatgtgaagt actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt 1141 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg 1201 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa 1261 tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt 1321 ttaaacgagc tcgaattcat cgatgatatc agatccacta gtggcctatg cggccgcgga 1381 tctgccggtc tccctatagt gagtcgtatt aatttcgata agccaggtta acctgcatta 1441 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 1501 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 1561 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 1621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 1681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 1741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 1801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 1861 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 1921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 1981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 2041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 2101 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 2161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 2221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 2281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 2341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 2401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 2461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 2521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 2581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 2641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 2701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 2761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 2821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 2881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 2941 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 3001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 3061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 3121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 3181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 3241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 3301 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 3361 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 3421 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 3481 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 3541 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 3601 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 3661 gagagtgcac catatggaca tattgtcgtt agaacgcggc tacaattaat acataacctt 3721 atgtatcata cacatacgat ttaggtgaca ctatagaacg cggccgccag ctgaagcttc 3781 gtacgctgca ggtcgacgga tcggtgacgg tgctggttta attaacatgt ctaaaggtga 3841 agaattattc actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca 3901 caaattttct gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa 3961 atttatttgt actactggta aattgccagt tccatggcca accttagtca ctactttagg 4021 ttatggtttg atgtgttttg ctagataccc agatcatatg aaacaacatg actttttcaa 4081 gtctgccatg ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa 4141 ctacaagacc agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt 4201 aaaaggtatt gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta 4261 taactctcac aatgtttaca tcatggctga caaacaaaag aatggtatca aagttaactt 4321 caaaattaga cacaacattg aagatggttc tgttcaatta gctgaccatt atcaacaaaa 4381 tactccaatt ggtgatggtc cagtcttgtt accagacaac cattacttat cctatcaatc 4441 tgccttatcc aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttgttac 4501 tgctgctggt attacccatg gtatggatga attgtacaaa cccgggttaa ttaacggtga 4561 acaaaagcta atctccgagg aagacttgaa cggtgaacaa aaattaatct cagaagaaga 4621 cttgaacgga ctcgacggtg aacaaaagtt gatttctgaa gaagatttga acggtgaaca 4681 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt 4741 gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa 4801 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa 4861 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct 4921 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg 4981 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat 5041 ctccgaggaa gacttgaacg gtgaacaaaa attaatcaat cactagtgaa ttcggcgcgc 5101 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa 5161 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt 5221 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagtatg aggtcgctct 5281 tattgaccac acctctaccg gcagatccgc tagggataac agggtaatat a //