LOCUS pKT212 4900 bp DNA CIRCULAR SYN 24-MAR-2010 DEFINITION ACCESSION pKT212 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4900) AUTHORS Self JOURNAL Unpublished. COMMENT SECNOTES|Mark A. Sheff and Kurt S. Thorn. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast 21 (2004) 661-670 FEATURES Location/Qualifiers misc_feature 322..407 /gene="ori" /product="origin of replication" CDS complement (1097..1957) /gene="AmpR" /product="bla, beta-lactamase" misc_feature 2469..2495 /gene="linker" /product="optimized linker" CDS 2496..3212 /gene="yEmCFP" /product="derived from yECFP (A206R)" misc_feature 3220..3422 /gene="ADH1t" /product="ADH1 terminator" misc_feature 3460..3838 /gene="TEFp" /product="Ashbya gossypii TEF promoter" CDS 3839..4651 /gene="CaURA3" /product="Candida albicans URA3, Orotidine-5'-phosphate (OMP) decarboxylase " misc_feature 4660..4884 /gene="TEFt" /product="Ashbya gossypii TEF terminator" BASE COUNT 1388 a 1048 c 1100 g 1364 t ORIGIN 1 cgaattcatc gatgatatca gatccactag tggcctatgc ggccgcggat ctgccggtct 61 ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc 121 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 181 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 241 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 301 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 361 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 421 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 481 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac 541 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 601 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 661 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 721 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 781 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 841 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 901 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 961 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 1021 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 1081 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 1141 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 1201 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 1261 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 1321 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 1381 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 1441 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 1501 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 1561 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 1621 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 1681 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 1741 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 1801 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 1861 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 1921 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 1981 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 2041 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 2101 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg 2161 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 2221 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 2281 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc 2341 atatggacat attgtcgtta gaacgcggct acaattaata cataacctta tgtatcatac 2401 acatacgatt taggtgacac tatagaacgc ggccgccagc tgaagcttcg tacgctgcag 2461 gtcgacggat cggtgacggt gctggtttaa ttaacatgtc taaaggtgaa gaattattca 2521 ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg 2581 tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta 2641 ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttaact tggggtgttc 2701 aatgtttttc tagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc 2761 cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca 2821 gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg 2881 attttaaaga agatggtaac attttaggtc acaaattgga atacatttat aactctcaca 2941 atgtttacat cactgctgac aaacaaaaga atggtatcaa agctaacttc aaaattagac 3001 acaacattga agatggttct gttcaattag ctgaccatta tcaacaaaat actccaattg 3061 gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct agattatcca 3121 aagatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact gctgctggta 3181 ttacccatgg tatggatgaa ttgtacaaat aaggcgcgcc acttctaaat aagcgaattt 3241 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt 3301 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta 3361 ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg 3421 cagatccgct agggataaca gggtaatata gatctgttta gcttgcctcg tccccgccgg 3481 gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt gacgtgcgca 3541 gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc catgtataat 3601 catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac ggctcctcgc 3661 tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt ccccacgccg 3721 cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc tttcatatac 3781 ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat aaacaaccat 3841 gacagtcaac actaagacct atagtgagag agcagaaact catgcctcac cagtagcaca 3901 gcgattattt cgattaatgg aactgaagaa aaccaattta tgtgcatcaa ttgacgttga 3961 taccactaag gaattccttg aattaattga taaattaggt ccttatgtat gcttaatcaa 4021 gactcatatt gatataatca atgatttttc ctatgaatcc actattgaac cattattaga 4081 actttcacgt aaacatcaat ttatgatttt tgaagataga aaatttgctg atattggtaa 4141 taccgtaaag aaacaatata ttggtggagt ttataaaatt agtagttggg cagatattac 4201 caatgctcat ggtgtcactg ggaatggagt ggttgaagga ttaaaacagg gagctaaaga 4261 aaccaccacc aaccaagagc caagagggtt attgatgtta gctgaattat catcagtggg 4321 atcattagca tatggagaat attctcaaaa aactgttgaa attgctaaat ccgataagga 4381 atttgttatt ggatttattg cccaacgtga tatgggtggc caagaagaag gatttgattg 4441 gcttattatg acacctggag ttggattaga tgataaaggt gatggattag gacaacaata 4501 tagaactgtt gatgaagttg ttagcactgg aactgatatt atcattgttg gtagaggatt 4561 gtttggtaaa ggaagagatc cagatattga aggtaaaagg tatagaaatg ctggttggaa 4621 tgcttatttg aaaaagactg gccaattata atcagtactg acaataaaaa gattcttgtt 4681 ttcaagaact tgtcatttgt atagtttttt tatattgtag ttgttctatt ttaatcaaat 4741 gttagcgtga tttatatttt ttttcgcctc gacatcatct gcccagatgc gaagttaagt 4801 gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg 4861 attcgatact aacgccgcca tccagtgtcg aaaacgagct //