LOCUS pKT101 4738 bp DNA CIRCULAR SYN 23-MAR-2010 DEFINITION pFA6a-link-yECFP-His5 ACCESSION pKT101 KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4738) AUTHORS Self JOURNAL Unpublished. COMMENT SECNOTES|Mark A. Sheff and Kurt S. Thorn. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast 21 (2004) 661-670 FEATURES Location/Qualifiers misc_feature 6..208 /gene="ADH1t" /product="ADH1 terminator" misc_feature 246..624 /gene="TEFp" /product="Ashbya gossypii TEF promoter" CDS 625..1278 /gene="SpHIS5" /product="Schizosaccharomyces pombe his5, imidazoleglycerol phosphate dehydratase" misc_feature 1287..1511 /gene="TEFt" /product="Ashbya gossypii TEF terminator" misc_feature 1846..1931 /gene="ori" /product="origin of replication" CDS complement (2621..3481) /gene="AmpR" /product="bla, beta-lactamase" misc_feature 3993..4019 /gene="linker" /product="optimized linker" CDS 4020..4736 /gene="yECFP" /product="derived from yEGFP1 (F64L, S65T, Y66W, N146I, M153T, V163A)" BASE COUNT 1282 a 1069 c 1079 g 1308 t ORIGIN 1 cgcgccactt ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt 61 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc 121 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gtatgaggtc 181 gctcttattg accacacctc taccggcaga tccgctaggg ataacagggt aatatagatc 241 tgtttagctt gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac 301 cctccttgac agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat 361 ttagcccata catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc 421 gcgaagcaaa aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac 481 agacgcgttg aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt 541 gccactgagg ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca 601 catcacatcc gaacataaac aaccatgggt aggagggctt ttgtagaaag aaatacgaac 661 gaaacgaaaa tcagcgttgc catcgctttg gacaaagctc ccttacctga agagtcgaat 721 tttattgatg aacttataac ttccaagcat gcaaaccaaa agggagaaca agtaatccaa 781 gtagacacgg gaattggatt cttggatcac atgtatcatg cactggctaa acatgcaggc 841 tggagcttac gactttactc aagaggtgat ttaatcatcg atgatcatca cactgcagaa 901 gatactgcta ttgcacttgg tattgcattc aagcaggcta tgggtaactt tgccggcgtt 961 aaaagatttg gacatgctta ttgtccactt gacgaagctc tttctagaag cgtagttgac 1021 ttgtcgggac ggccctatgc tgttatcgat ttgggattaa agcgtgaaaa ggttggggaa 1081 ttgtcctgtg aaatgatccc tcacttacta tattcctttt cggtagcagc tggaattact 1141 ttgcatgtta cctgcttata tggtagtaat gaccatcatc gtgctgaaag cgcttttaaa 1201 tctctggctg ttgccatgcg cgcggctact agtcttactg gaagttctga agtcccaagc 1261 acgaagggag tgttgtaaag agtactgaca ataaaaagat tcttgttttc aagaacttgt 1321 catttgtata gtttttttat attgtagttg ttctatttta atcaaatgtt agcgtgattt 1381 atattttttt tcgcctcgac atcatctgcc cagatgcgaa gttaagtgcg cagaaagtaa 1441 tatcatgcgt caatcgtatg tgaatgctgg tcgctatact gctgtcgatt cgatactaac 1501 gccgccatcc agtttaaacg agctcgaatt catcgatgat atcagatcca ctagtggcct 1561 atgcggccgc ggatctgccg gtctccctat agtgagtcgt attaatttcg ataagccagg 1621 ttaacctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 1681 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 1741 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 1801 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 1861 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 1921 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 1981 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 2041 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 2101 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 2161 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 2221 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 2281 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 2341 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 2401 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 2461 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 2521 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 2581 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 2641 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 2701 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 2761 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 2821 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 2881 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 2941 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 3001 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 3061 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 3121 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 3181 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 3241 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 3301 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 3361 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 3421 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 3481 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 3541 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 3601 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 3661 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 3721 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 3781 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 3841 agcagattgt actgagagtg caccatatgg acatattgtc gttagaacgc ggctacaatt 3901 aatacataac cttatgtatc atacacatac gatttaggtg acactataga acgcggccgc 3961 cagctgaagc ttcgtacgct gcaggtcgac ggatcggtga cggtgctggt ttaattaaca 4021 tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa ttagatggtg 4081 atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct acttacggta 4141 aattgacctt aaaatttatt tgtactactg gtaaattgcc agttccatgg ccaaccttag 4201 tcactacttt aacttggggt gttcaatgtt tttctagata cccagatcat atgaaacaac 4261 atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca 4321 aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta 4381 atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat 4441 tggaatacat ttataactct cacaatgttt acatcactgc tgacaaacaa aagaatggta 4501 tcaaagctaa cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa ttagctgacc 4561 attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact 4621 tatccactca atctgcctta tccaaagatc caaacgaaaa gagagaccac atggtcttgt 4681 tagaatttgt tactgctgct ggtattaccc atggtatgga tgaattgtac aaataagg //