| EUROSCARF
|
A versatile toolbox for PCR-based tagging of yeast genes:
new fluorescent proteins, more markers and promoter substitution cassettes
Carsten Janke, Maria M. Magiera, Nicole Rathfelder, Christof Taxis, Simone Reber, Hiromi Maekawa,
Alexandra Moreno-Borchart, Georg Doenges, Etienne Schwob, Elmar Schiebel, Michael Knop
Yeast (2004) 21, 947 - 962
We offer all plasmids listed in the above paper as a kit (Acc. Nr.: PCR-TOOLBOX) containing appr. 75 plasmids
for a handling fee of 200 EUR plus an administration fee of 15 EUR per order. Several months behind shedule
we now start to distribute the plasmids. The use of the plasmids is restricted to academical users for pure academic research.
Before dispatching the plasmids we need a signed material transfer agreement. Please download
the material
transfer agreement, and send two signed copies to EUROSCARF. We will return one countersigned material transfer agreement
together with the plasmids.
When applying for the plasmids pYM48 and pYM51,
additional material transfer agreements have to be signed. Plasmids pYM37 and pYM41 did not
pass our quality check. The constructors were not able to replace the incorrect plasmids.
| accession no. |
plasmid |
tag |
marker |
| P30347 |
pFA6-hphNT1 |
- |
hphNT1 |
| P30346 |
pFA6-natNT2 |
- |
natNT2 |
| P30299 |
pYM13 |
TAP |
kanMX4 |
| P30300 |
pYM14 |
6HA |
kanMX4 |
| P30301 |
pYM15 |
6HA |
HIS3MX6 |
| P30302 |
pYM16 |
6HA |
hphNT1 |
| P30303 |
pYM17 |
6HA |
natNT2 |
| P30304 |
pYM18 |
9myc |
kanMX4 |
| P30305 |
pYM19 |
9myc |
HIS3MX6 |
| P30306 |
pYM20 |
9myc |
hphNT1 |
| P30307 |
pYM21 |
9myc |
natNT2 |
| P30308 |
pYM22 |
3HA |
klTRP1 |
| P30235 |
pYM23 |
3myc |
klTRP1 |
| P30236 |
pYM24 |
3HA |
hphNT1 |
| P30237 |
pYM25 |
yeGFP |
hphNT1 |
| P30238 |
pYM26 |
yeGFP |
klTRP1 |
| P30239 |
pYM27 |
EGFP |
kanMX4 |
| P30240 |
pYM28 |
EGFP |
HIS3MX6 |
| P30241 |
pYM29 |
EGFP |
klTRP1 |
| P30242 |
pYM30 |
ECFP |
kanMX4 |
| P30243 |
pYM31 |
ECFP |
HIS3MX6 |
| P30244 |
pYM32 |
ECFP |
klTRP1 |
| P30245 |
pYM33 |
EBFP |
kanMX4 |
| P30246 |
pYM34 |
EBFP |
klTRP1 |
| P30247 |
pYM35 |
DsRED1 |
kanMX4 |
| P30248 |
pYM36 |
DsRED1 |
klTRP1 |
| P30250 |
pYM38 |
RedStar |
kanMX4 |
| P30251 |
pYM39 |
EYFP |
kanMX4 |
| P30252 |
pYM40 |
EYFP |
hphNT1 |
| P30254 |
pYM42 |
RedStar* |
natNT2 |
| P30255 |
pYM43 |
RedStar2 |
natNT2 |
| P30256 |
pYM44 |
yeGFP |
HIS3MX6 |
| P30257 |
pYM45 |
1HA |
kanMX4 |
| P30258 |
pYM46 |
1myc-7HIS |
kanMX4 |
| P30259 |
pYM47 |
FlAsH |
hphNT1 |
| P30260 |
pYM48 |
PA-GFP (additional MTA required) |
hphNT1 |
| P30261 |
pYM51 |
eqFP611 (additional MTA required) |
hphNT1 |
| P30262 |
pYM-N1 |
CUP1 Promoter, - |
kanMX4 |
| P30263 |
pYM-N2 |
CUP1 Promoter, - |
natNT2 |
| P30264 |
pYM-N3 |
CUP1 Promoter, 3HA |
natNT2 |
| P30265 |
pYM-N4 |
CUP1 Promoter, yeGFP |
natNT2 |
| P30266 |
pYM-N5 |
CUP1 Promoter, ProA |
natNT2 |
| P30267 |
pYM-N6 |
ADH Promoter, - |
kanMX4 |
| P30268 |
pYM-N7 |
ADH Promoter, - |
natNT2 |
| P30289 |
pYM-N8 |
ADH Promoter, 3HA |
natNT2 |
| P30269 |
pYM-N9 |
ADH Promoter, yeGFP |
natNT2 |
| P30290 |
pYM-N10 |
CYC1 Promoter, - |
kanMX4 |
| P30291 |
pYM-N11 |
CYC1 Promoter, - |
natNT2 |
| P30292 |
pYM-N12 |
CYC1 Promoter, 3HA |
natNT2 |
| P30270 |
pYM-N13 |
CYC1 Promoter, yeGFP |
natNT2 |
| P30271 |
pYM-N14 |
GPD Promoter, - |
kanMX4 |
| P30272 |
pYM-N15 |
GPD Promoter, - |
natNT2 |
| P30293 |
pYM-N16 |
GPD Promoter, 3HA |
natNT2 |
| P30273 |
pYM-N17 |
GPD Promoter, yeGFP |
natNT2 |
| P30274 |
pYM-N18 |
TEF Promoter, - |
kanMX4 |
| P30275 |
pYM-N19 |
TEF Promoter, - |
natNT2 |
| P30294 |
pYM-N20 |
TEF Promoter, 3HA |
natNT2 |
| P30276 |
pYM-N21 |
TEF Promoter, yeGFP |
natNT2 |
| P30277 |
pYM-N22 |
GAL1 Promoter, - |
kanMX4 |
| P30278 |
pYM-N23 |
GAL1 Promoter, - |
natNT2 |
| P30295 |
pYM-N24 |
GAL1 Promoter, 3HA |
natNT2 |
| P30279 |
pYM-N25 |
GAL1 Promoter, yeGFP |
natNT2 |
| P30280 |
pYM-N26 |
GALL Promoter, - |
kanMX4 |
| P30281 |
pYM-N27 |
GALL Promoter, - |
natNT2 |
| P30296 |
pYM-N28 |
GALL Promoter, 3HA |
natNT2 |
| P30282 |
pYM-N29 |
GALL Promoter, yeGFP |
natNT2 |
| P30283 |
pYM-N30 |
GALS Promoter, - |
kanMX4 |
| P30284 |
pYM-N31 |
GALS Promoter, - |
natNT2 |
| P30297 |
pYM-N32 |
GALS Promoter, 3HA |
natNT2 |
| P30285 |
pYM-N33 |
GALS Promoter, yeGFP |
natNT2 |
| P30286 |
pYM-N34 |
MET25 Promoter, - |
kanMX4 |
| P30287 |
pYM-N35 |
MET25 Promoter, - |
natNT2 |
| P30298 |
pYM-N36 |
MET25 Promoter, 3HA |
natNT2 |
| P30288 |
pYM-N37 |
MET25 Promoter, yeGFP |
natNT2 |
30-Jun-2005
|