EUROpean Saccharomyces Cerevisiae

ARchive for Functional Analysis
 
Bimolecular fluorescence complementation analysis system for in vivo detection of protein-protein interaction in Saccharomyces cerevisiae

Min-Kyung Sung and Won-Ki Huh

Yeast 2007 24: 765 - 775

Plasmids for studying in vivo detection of protein-protein interactions by bimolecular fluorescence complementation (BiFC) assays. The created plasmids allow single-step, PCR-based C- or N-terminal tagging of yeast proteins with yellow fluorescence protein fragments for BiFC assay.
VN: N-terminal fragment of Venus
VC: C-terminal fragment of Venus

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Due to discrepancies found by restriction analysis plasmids containing the HIS3MX6 marker cassette were partially re-sequenced. By this it was obviously that the origin of the HIS3 was not Schizosaccharomyces pombe but Saccharomyces klyuveri.
[EUROSCARF, Peter Kötter, 19-01-2010]

The old plasmids (acc.-no. P30567 - P30572) containing the RPL7B promoter instead CET1 promoter were replaced .
[EUROSCARF, Peter Kötter, 08-10-2012]

Plasmids for C-terminal tagging:
accession no. plasmid name E.coli
transformation marker		 comments
P30555 pFA6a-VN-HIS3MX6 AmpR map and sequence file changed 19-01-2010
P30556 pFA6a-VC-HIS3MX6 AmpR map and sequence file changed 19-01-2010
P30557 pFA6a-VN-TRP1 AmpR  
P30558 pFA6a-VC-TRP1 AmpR  
P30559 pFA6a-VN-kanMX6 AmpR  
P30560 pFA6a-VC-kanMX6 AmpR  

Plamids for N-terminal tagging:
accession no. plasmid name E.coli
transformation marker		 comments
P30561 pFA6a-HIS3MX6-pGAL1-VN AmpR map and sequence file changed 19-01-2010
P30562 pFA6a-HIS3MX6-pGAL1-VC AmpR map and sequence file changed 19-01-2010
P30563 pFA6a-TRP1-pGAL1-VN AmpR  
P30564 pFA6a-TRP1-pGAL1-VC AmpR  
P30565 pFA6a-kanMX6-pGAL1-VN AmpR  
P30566 pFA6a-kanMX6-pGAL1-VC AmpR  
P30662 pFA6a-HIS3MX6-pCET1-VN AmpR map and sequence file changed 19-01-2010
the old version (P30567) contains the RPL7B promoter instead of the CET1 promoter, replaced on 08-10-2012
P30663 pFA6a-HIS3MX6-pCET1-VC AmpR map and sequence file changed 19-01-2010
the old version (P30568) contains the RPL7B promoter instead of the CET1 promoter, replaced on 08-10-2012
P30664 pFA6a-TRP1-pCET1-VN AmpR the old version (P30569) contains the RPL7B promoter instead of the CET1 promoter, replaced on 08-10-2012
P30665 pFA6a-TRP1-pCET1-VC AmpR the old version (P30570) contains the RPL7B promoter instead of the CET1 promoter, replaced on 08-10-2012
P30666 pFA6a-kanMX6-pCET1-VN AmpR the old version (P30571) contains the RPL7B promoter instead of the CET1 promoter, replaced on 08-10-2012
P30667 pFA6a-kanMX6-pCET1-VC AmpR the old version (P30568) contains the RPL7B promoter instead of the CET1 promoter, replaced on 08-10-2012

  17-January-2013
MIPS 

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