EUROpean Saccharomyces Cerevisiae

ARchive for Functional Analysis
 
Cytosine deaminase MX cassettes as positive/negative selectable markers in Saccharomyces cerevisiae

Phillip E. Hartzog, Bradly P. Nicholson and John H. McCusker

Yeast (2005) 22: 789 - 798

Positive/negative selectable cytosine deaminase MX cassettes for use in Saccharomyces cerevisiae. The basis of positive selection for cytosine deaminase (Fcy1) activity is that  fcy1 strains are unable to grow on medium containing cytosine as a sole nitrogen source and fcy1 ura3 strains are unable to grow on medium containing cytosine as the sole pyrimidine source. Conversely, as 5-fluorocytosine (5FC) is toxic to cytosine deaminase-producing cells, fcy1 strains are resistant to 5FC.

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accession no. plasmid name S. cerevisiae dominant marker E.coli
transformation marker
P30399 pPH34 FCA1MX3 AmpR
P30400 pPH35 FCY1MX3 AmpR
P30401 pPH36 FCA1MX4 AmpR
P30402 pPH37 FCY1MX4 AmpR
P30403 pBN42 kanMX4-pefA-DR AmpR
P30404 pBN62 FCA1MX4-pefA-DR AmpR
P30405 pBN63 FCY1MX4-pefA-DR AmpR
P30406 pBN100 CaURA3MX4-pefA-DR AmpR
P30407 pBN27 FCY1 AmpR
P30408 pBN50 kanMX4-pefA-DR,pRS314 AmpR
P30409 pBN52 FCA1MX4-pefA-DR,pRS314 AmpR
P30410 pBN53 FCY1MX4-pefA-DR,pRS314 AmpR
P30411 pBN97 CaURA3MX4-pefA-DR,pRS314 AmpR
 
  11-September-2009
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