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EUROpean
Saccharomyces Cerevisiae
ARchive for Functional Analysis |
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Cytosine deaminase MX cassettes as
positive/negative selectable markers in
Saccharomyces cerevisiae
Phillip E. Hartzog, Bradly P. Nicholson and John H. McCusker Positive/negative selectable cytosine deaminase MX cassettes for use in Saccharomyces cerevisiae. The basis of positive selection for cytosine deaminase (Fcy1) activity is that fcy1 strains are unable to grow on medium containing cytosine as a sole nitrogen source and fcy1 ura3 strains are unable to grow on medium containing cytosine as the sole pyrimidine source. Conversely, as 5-fluorocytosine (5FC) is toxic to cytosine deaminase-producing cells, fcy1 strains are resistant to 5FC. Please find additional information concerning ordering, shipment, and invoicing under ordering conditions.
11-September-2009
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