ARchive for Functional Analysis
Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae
Mark A. Sheff and Kurt S. Thorn
The use of green fluorescent protein (GFP) as a protein tag has become increasingly popular for determining protein localization and abundance. With the availability of GFP variants with altered fluorescence spectra, as well as GFP homologues from other organisms, multi-color fluorescence with protein tags is now possible, as is measuring protein interactions using fluorescence resonance energy transfer (FRET). This set of yeast tagging vectors contains codon optimized variants of GFP, CFP (cyan), YFP (yellow), and Sapphire (a UV-excitable GFP). These codon optimized tags are twice as detectable as unoptimized tags.
All users of the tdimer2 plasmids must sign an MTA with Dr. Roger Tsien (UCSD) before obtaining the plasmids.
Due to low ordering frequency the "GFP-PCR-SET1" and "GFP-PCR-SET2" with a reduced handling fee will no longer be dispatched.
The individual plasmids are still available. Please order by individual accession numbers. You will be billed for each plasmid, as given in the handling fee list under ordering conditions.