ARchive for Functional Analysis
A new series of yeast shuttle vectors for the recovery and identiﬁcation of multiple plasmids from Saccharomyces cerevisiae
LilyAnn Novak Frazer and Raymond T. O’Keefe
To facilitate the
recovery and identiﬁcation of multiple plasmids from S. cerevisiae,
a series of plasmids based on the pRS series of yeast shuttle vectors has
been constructed. Bacterial antibiotic resistance genes to chloramphenicol,
kanamycin and zeocin have been combined with the yeast centromere sequence
(CEN6), the autonomously replicating sequence (ARSH4) and
one of the four yeast selectable marker genes (HIS3, TRP1,
LEU2 or URA3) from the pRS series of vectors.
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