Deletion Marker Plasmids

EUROpean Saccharomyces Cerevisiae

ARchive for Functional Analysis

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Deletion Marker Plasmids

This is a collection of plasmids useful in gene deletion experiments. These plasmids have been provided by Alan Goldstein and John McCusker and by Hans Hegemann and coworkers. For referencing purposes please use the publications mentioned.

Please find additional information concerning ordering, shipment, and invoicing under ordering conditions.

new dominant marker gene deletion cassettes

accession no.	plasmid name	marker gene	selectable phenotype	reference
P30104	pAG25	P_AgTEF1-natMX-T_AgTEF1	clonNAT^R	1
P30105	pAG29	P_AgTEF1-patMX-T_AgTEF1	Bialaphos^R	1
P30106	pAG32	P_AgTEF1-hphMX-T_AgTEF1	Hygromycin B^R	1
P30107	pAG35	dr-P_AgTEF1-natMX-T_AgTEF1-dr	clonNAT^R	1
P30108	pAG31	dr-P_AgTEF1-patMX-T_AgTEF1-dr	Bialaphos^R	1
P30109	pAG34	dr-P_AgTEF1-hphMX-T_AgTEF1-dr	Hygromycin B^R	1

new dominant marker gene deletion cassettes in yeast/E. coli vector pRS316 (CEN/ARS, URA3)

accession no.	plasmid name	marker gene	selectable phenotype	reference
P30110	pAG36	P_AgTEF1-natMX-T_AgTEF1	clonNAT^R, URA3	1
P30113	pAG26	P_AgTEF1-hphMX-T_AgTEF1	Hygromycin B^R, URA3	1

CaURA3 marker gene deletion cassettes

accession no.	plasmid name	marker gene	selectable phenotype	reference
P30111	pAG60	P_AgTEF1-CaURA3-T_AgTEF1	URA⁺	2
P30112	pAG61	dr-P_AgTEF1-CaURA3-T_AgTEF1-dr	URA⁺	2

loxP-flanked marker gene deletion cassettes

accession no.	plasmid name	marker gene	selectable phenotype	reference
P30114	pUG6	loxP-P_AgTEF1-kanMX-T_AgTEF1-loxP	G418^R	3
P30115	pUG27	loxP-P_AgTEF1-Sphis5-T_AgTEF1-loxP	HIS⁺	4
P30116	pUG66	loxP-P_AgTEF1-ble-T_AgTEF1-loxP	Phleo^R	4
P30117	pUG72	loxP-P_KlURA3-KlURA3-T_KlURA3-loxP	URA⁺	4
P30118	pUG73	loxP-P_KlLEU2-KlLEU2-T_KlLEU2-loxP	LEU⁺	4
P30670	pUG74	loxP-P_AgTEF1-natMX-T_AgTEF1-loxP	clonNAT^R	5 new
P30671	pUG75	loxP-P_AgTEF1-hphMX-T_AgTEF1-loxP	Hygromycin B^R	5 new

Cre-expressing (P_GAL1-cre) CEN/ARS plasmids

accession no.	plasmid name	marker gene	selectable phenotype	reference
P30119	pSH47	P_ScURA3-ScURA3-T_ScURA3	URA⁺	3
P30120	pSH62	P_ScHIS3-ScHIS3-T_ScHIS3	HIS⁺	4
P30121	pSH63	P_ScTRP1-ScTRP1-T_ScTRP1	TRP⁺	4
P30122	pSH65	P_AgTEF1-ble-T_ScCYC1	Phleo^R	4
P30672	pSH66	P_AgTEF1-natMX-T_ScCYC1	clonNAT^R	5 new
P30673	pSH67	P_AgTEF1-kanMX-T_ScCYC1	G418^R	5 new
P30674	pSH68	P_ScHIS3-ScLEU2-T_ScHIS3	LEU⁺	5 new
P30675	pSH69	P_AgTEF1-hphMX-T_ScCYC1	Hygromycin B^R	5 new

P: promoter; T: terminator; dr: direkt repeat; Ag: Ashbya gossypii; Sc: Saccharomyces cerevisiae; Kl: Kluyveromyces lactis; Sp: Schizosaccharomyces cerevisiae; Ca: Candida albicans

References:

1) Goldstein, A.L., and McCusker, J.H. 1999: Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15 , 1541-1553 [Pubmed]

2) Goldstein, A.L., Pan, X., and McCusker, J.H. 1999: Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae. Yeast 15, 507-511 [Pubmed]

3) Güldener, U., Heck, S., Fiedler, T., Beinhauer, J., and Hegemann, J.H. 1996: A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Research 24, 2519-2524 [Pubmed]

4) Güldener, U., Heinisch, J., Köhler G.J., Voss, D., and Hegemann, J.H. 2002: A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Research 30, e23 [Pubmed]

5) Hegemann, J.H. and Heick, S.B. 2011. Delete and repeat: a comprehensive toolkit for sequential gene knockout in the budding yeast Saccharomyces cerevisiae. Methods Mol Biol 765, 189-206 [Pubmed]

28-September-2012

Peter Kötter

MIPS

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