EUROpean Saccharomyces Cerevisiae

ARchive for Functional Analysis
 
Deletion Marker Plasmids

This is a collection of plasmids useful in gene deletion experiments. These plasmids have been provided by Alan Goldstein and John McCusker and by Hans Hegemann and coworkers. For referencing purposes please use the publications mentioned. 

Please find additional information concerning ordering, shipment, and invoicing  under ordering conditions.

new dominant marker gene deletion cassettes

accession no. plasmid name marker gene selectable phenotype reference
P30104 pAG25 PAgTEF1-natMX-TAgTEF1 clonNATR 1
P30105 pAG29 PAgTEF1-patMX-TAgTEF1 BialaphosR 1
P30106 pAG32 PAgTEF1-hphMX-TAgTEF1 Hygromycin BR 1
P30107 pAG35 dr-PAgTEF1-natMX-TAgTEF1-dr clonNATR 1
P30108 pAG31 dr-PAgTEF1-patMX-TAgTEF1-dr BialaphosR 1
P30109 pAG34 dr-PAgTEF1-hphMX-TAgTEF1-dr Hygromycin BR 1

 

new dominant marker gene deletion cassettes in yeast/E. coli vector pRS316 (CEN/ARS, URA3)

accession no. plasmid name marker gene selectable phenotype reference
P30110 pAG36 PAgTEF1-natMX-TAgTEF1 clonNATR, URA3 1
P30113 pAG26 PAgTEF1-hphMX-TAgTEF1 Hygromycin BR, URA3 1

 

CaURA3 marker gene deletion cassettes

accession no. plasmid name marker gene selectable phenotype reference
P30111 pAG60 PAgTEF1-CaURA3-TAgTEF1 URA+ 2
P30112 pAG61 dr-PAgTEF1-CaURA3-TAgTEF1-dr URA+ 2

 

loxP-flanked marker gene deletion cassettes

accession no. plasmid name marker gene selectable phenotype reference
P30114 pUG6 loxP-PAgTEF1-kanMX-TAgTEF1-loxP G418R 3
P30115 pUG27 loxP-PAgTEF1-Sphis5-TAgTEF1-loxP HIS+ 4
P30116 pUG66 loxP-PAgTEF1-ble-TAgTEF1-loxP PhleoR 4
P30117 pUG72 loxP-PKlURA3-KlURA3-TKlURA3-loxP URA+ 4
P30118 pUG73 loxP-PKlLEU2-KlLEU2-TKlLEU2-loxP LEU+ 4
P30670 pUG74 loxP-PAgTEF1-natMX-TAgTEF1-loxP clonNATR 5 new
P30671 pUG75 loxP-PAgTEF1-hphMX-TAgTEF1-loxP Hygromycin BR 5 new

 

Cre-expressing (PGAL1-cre) CEN/ARS plasmids

accession no. plasmid name marker gene selectable phenotype reference
P30119 pSH47 PScURA3-ScURA3-TScURA3 URA+ 3
P30120 pSH62 PScHIS3-ScHIS3-TScHIS3 HIS+ 4
P30121 pSH63 PScTRP1-ScTRP1-TScTRP1 TRP+ 4
P30122 pSH65 PAgTEF1-ble-TScCYC1 PhleoR 4
P30672 pSH66 PAgTEF1-natMX-TScCYC1 clonNATR 5 new
P30673 pSH67 PAgTEF1-kanMX-TScCYC1 G418R 5 new
P30674 pSH68 PScHIS3-ScLEU2-TScHIS3 LEU+ 5 new
P30675 pSH69 PAgTEF1-hphMX-TScCYC1 Hygromycin BR 5 new
P: promoter; T: terminator; dr: direkt repeat; Ag: Ashbya gossypii; Sc: Saccharomyces cerevisiae; Kl: Kluyveromyces lactis; Sp: Schizosaccharomyces cerevisiae; Ca: Candida albicans

References:

1) Goldstein, A.L., and McCusker, J.H. 1999: Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15 , 1541-1553 [Pubmed]

2) Goldstein, A.L., Pan, X., and McCusker, J.H. 1999: Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae. Yeast 15, 507-511 [Pubmed]

3) Güldener, U., Heck, S., Fiedler, T., Beinhauer, J., and Hegemann, J.H. 1996: A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Research 24, 2519-2524 [Pubmed]

4) Güldener, U., Heinisch, J., Köhler G.J., Voss, D., and Hegemann, J.H. 2002: A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Research 30, e23 [Pubmed]

5) Hegemann, J.H. and Heick, S.B. 2011. Delete and repeat: a comprehensive toolkit for sequential gene knockout in the budding yeast Saccharomyces cerevisiae. Methods Mol Biol 765, 189-206 [Pubmed]

  28-September-2012
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