EUROPEAN
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EUROSCARF

TAP Plasmids

Please find a link to the description of the methods and protocols for using the TAP plasmids by B. Seraphin.


Yeast expression plasmids:

Accession number: pBS1479 (sequence)

    pBS1479 (plasmid for TAP-tagging of proteins at the C-terminus.
      Rigaut, G; Shevchenko, A; Rutz, B.; Wilm, M.; Mann, M.; Seraphin, B. (1999), Nature Biotechnology. Vol. 17 (10): 1030-1032)

Accession number: pBS1539 (Sequence)

    pBS1539 (plasmid based on pBS1479 containing an K. lactis URA3 marker instead of K. lactis TRP1.

      Puig, O; Caspary, F; Rigaut, G; Rutz, B.; Bouveret, E; Bragado-Nilsson, E; Wilm, M; Seraphin, B. (2001), Methods. Vol 24: 218-229)

Accession number: pBS1761 (Sequence)

    pBS1761 (plasmid based on pBS1479 for TAP-tagging of proteins at the N-terminus.
      Seraphin, B; Puig, O; Bouveret, E; Rutz, B; Caspary, F. Cold Spring Harbor Laboratory A Molecular Cloning Manual: Protein-Protein Interactions. Golermis, E. and Serebriiskii, I. editors. Cold Spring Harbor Laboratory Press, Chapter 17, 313-328)


Mammalian expression plasmids:

Accession number: pZome1C (Sequence)

    pZome1-C (plasmid based on pBabe-puro (Morgenstern, JP; Land, H. (1990), Nucl. Acid Res. 18: 3587-3596) containing the TAP-tag (Rigaut, G; Shevchenko, A; Rutz, B.; Wilm, M.; Mann, M.; Seraphin, B. (1999), Nature Biotechnology. Vol. 17 (10): 1030-1032) for TAP-tagging proteins at the C-terminus)

Accession number: pZome1N (Sequence)

    pZome1-N (plasmid based on pBabe-puro (Morgenstern, JP; Land, H. (1990), Nucl. Acid Res. 18: 3587-3596) containing the TAP-tag (Rigaut, G; Shevchenko, A; Rutz, B.; Wilm, M.; Mann, M.; Seraphin, B. (1999), Nature Biotechnology. Vol. 17 (10): 1030-1032) for TAP-tagging proteins at the N-terminus

  • TAP fusion yeast strains available from EUROSCARF
  • Material Transfer Agreement, Version for German Recipients
  • How to order TAP plasmids from EUROSCARF

  • 21-Feb-2003
    Matthias Rose
    MIPS

    SGD