EUROpean Saccharomyces Cerevisiae

ARchive for Functional Analysis

yeast strains


how to order



Fast protein-depletion system utilizing tetracycline repressible promoter and N-end rule in yeast.
Gnanasundram SV and Kos M. Mol Biol Cell (2015) 26:762-768


CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.
Mans R, van Rossum HM, Wijsman M, Backx A, Kuijpers NGA, van den Broek M, Daran-Lapujade P, Pronk JT, van Maris AJA, Daran JMG. FEMS Yeast Res, 2015


Systematic exploration of essential yeast gene function with temperature-sensitive mutants.
Li Z, Vizeacoumar FJ, Bahr S, Li J, Warringer J, Vizeacoumar FS, Min R, Vandersluis B, Bellay J, Devit M, Fleming JA, Stephens A, Haase J, Lin ZY, Baryshnikova A, Lu H, Yan Z, Jin K, Barker S, Datti A, Giaever G, Nislow C, Bulawa C, Myers CL, Costanzo M, Gingras AC, Zhang Z, Blomberg A, Bloom K, Andrews B, Boone C. Nat Biotechnol. 2011 (29) 361-367

available soon

pREPORT: a multi-readout transcription reporter vector for fission yeast
Martin Prevorovsky. Yeast (2015) 32:327-334

One-step assembly and targeted integration of multigene constructs assisted by the I-SceI meganuclease inSaccharomyces cerevisiae

Kuijpers NG, Chroumpi S, Vos T, Solis-Escalante D, Bosman L, Pronk JT, Daran JM and Daran-Lapujade P . FEMS Yeast Res (2013) 13:769-781

Efficient simultaneous excision of multiple selectable marker cassettes using I-SceI-induced double-strand breaks in Saccharomyces cerevisiae
Solis-Escalante D, Kuijpers NG, van der Linden FH, Pronk JT, Daran JM and Daran-Lapujade P. FEMS Yeast Res (2014) 14:741-754

The MX4blaster cassette: repeated and clean Saccharomyces cerevisiae genome modification using the genome-wide deletion collection
Angela Carvalho, Filipa Pereira & Bjorn Johansson. FEMS Yeast Res (2013) 13:711-719

A prototrophic deletion mutant collection for yeast metabolomics and systems biology
Mülleder M, Capuano F, Pir P, Christen S, Sauer U, Oliver SG and Ralser M  Nat Biotechnol. (2012) 30:1176-8
complete mutant collection available 22-02-2013

A new versatile system for rapid control of gene expression in the fission yeast Schizosaccharomyces pombe
Zilio N, Wehrkamp-Richter S and Boddy MN  Yeast (2012) 10: 425-34

103 plasmids deposited by EMBL scientists around the group of Jan Ellenberg
1 new plasmid available  19-10-2009
7 new plasmids available  31-08-2009
19 new plasmids available    15-01-2010
3 new plasmids available    01-02-2011

3 new plasmids available    25-08-2011
3 new plasmids available    15-01-2013

amdSYM, a recyclable and counterselectable dominant marker new
Daniel Solis-Escalante, Niels G. A. Kuijpers, Nadine Bongaerts, Irina Bolat, LizanneBosman, Jack T. Pronk, Jean-Marc Daranand Pascale Daran-Lapujade. FEMS Yeast Research (2012) doi: 10.1111/1567-1364.12024

Deletion marker plasmids

now all plasmid available described in:
Hegemann, J.H. and Heick, S.B. 2011. Delete and repeat: a comprehensive toolkit for sequential gene knockout in the budding yeast Saccharomyces cerevisiae. Methods Mol Biol 765, 189-206

Homoserine toxicity in Saccharomyces cerevisiae and Candida albicans homoserine kinase (thr1Delta) mutants
Joanne M. Kingsbury and John McCusker.
Eukaryot Cell (2010) 9: 717-728

System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae
Christof Taxis and Michael Knop. Biotechniques  (2006) 40, 73-77

A new series of yeast shuttle vectors for the recovery and identification of multiple plasmids from Saccharomyces cerevisiae
LilyAnn Novak Frazer and Raymond T. O’Keefe. Yeast (2007) 24, 777-789

A fast and efficient translational control system for conditional expression of yeast genes
Kötter P, Weigand JE, Meyer B, Entian K-D, Suess B. NAR (2009) 37, e120 

Dominant marker vectors for selecting yeast mating products
Ivan Sadowski, Pedro Lourenco and Jennifer Parent . Yeast (2008) 25, 595-599 

Cytosine deaminase MX cassettes as positive/negative selectable markers in Saccharomyces cerevisiae
Phillip E. Hartzog, Bradly P. Nicholson and John H. McCusker. Yeast (2005) 22, 789-798 

Bimolecular fluorescence complementation analysis system for in vivo detection of protein-protein interaction in Saccharomyces cerevisiae
Min-Kyung Sung and Won-Ki Huh. Yeast (2007) 24, 767-775 

Vectors for N- or C-terminal positioning of the yeast Gal4p DNA binding or activator domains
Stefan H Millson, Andrew W Truman and Peter P Piper. BioTechniques (2003) 35: 60-64

Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe
Watson AT, Garcia V, Bone N, Carr AM, Armstrong J. Gene (2008) 407, 63 - 74

The Anchor-Away Technique: Rapid, Conditional Establishment of Yeast Mutant Phenotypes
Haruki H, Nishikawa J, Laemmli UK. Mol Cell (2008) 31, 925 - 932

An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
T Bürckstümmer, KL Bennett, A Preradovic, G Schütze, O Hantschel, G Superti-Furga, A Bauch. Nature Methods (2006) 3, 1013-1019

An improved tetO promoter replacement system for regulating the expression of yeast genes
Yen K, Gitsham P, Wishart J, Oliver SG, Zhang N. Yeast (2003) 20, 1255 - 1262

Disintegrator vectors for single-copy yeast chromosomal integration
Ivan Sadowski, Ting-Cheng Su and Jennifer Parent. Yeast (2007) 24, 447 - 455

Almost complete yeast TAP fusion protein strain set available
Gavin et al., Nature (2006) 440, 631 - 636.

Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe
Pierre Hentges, Benoit Van Driessche, Lionel Tafforeau, Jean Vandenhoute and Anthony M. Carr. Yeast (2005) 22, 1013 - 1019

Additional vectors for PCR-based gene tagging in Saccharomyces cerevisiae and Schizosaccharomyces pombe using nourseothicin resistance
Benoit Van Driessche, Lionel Tafforeau, Pierre Hentges, Anthony M. Carr and Jean Vandenhoute. Yeast (2005) 22, 1061 - 1068

Drag & Drop Cloning in Yeast.
Gregor Jansen, Cunle Wu, Babette Schade, David Y. Thomas, Malcolm Whiteway. Gene (2005) 344, 43 - 51

New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae
Robert Gauss, Mark Trautwein, Thomas Sommer, Anne Spang. Yeast (2005) 22, 1 - 12

A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes
Carsten Janke, Maria M. Magiera, Nicole Rathfelder, Christof Taxis, Simone Reber, Hiromi Maekawa, Alexandra Moreno-Borchart, Georg Doenges, Etienne Schwob, Elmar Schiebel, Michael Knop. Yeast (2004) 21, 974 - 962

Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae
Mark A. Sheff and Kurt S. Thorn. Yeast (2004) 21, 661 - 670

LYS5 marker cassettes
Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae.
Sayoko Ito-Harashima and John McCusker. Yeast (2004) 21, 53 - 61

DsdA (D-serine deaminase): a new heterologous MX cassette of gene disruption and selection in Saccharomyces cerevisiae
Mara K. Vorachek-Warren and John McCusker. Yeast (2004) 21, 163 - 171

588 Yeast TAP fusion protein strains and TAP plasmids available
Functional organization of the yeast proteome by systematic analysis of protein complexes.
Gavin et al., Nature (2002) 415, 141-147.

Kit for making CUP1 t.s. degrons by PCR
Kanamaki, M., Sanchez-Diaz, A., Gambus, A., and Labib, K., Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature (2003) 423, 720 - 724.

New: One hundred finished yeast degron strains available.

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Peter Kötter