EUROSCARF

EUROpean Saccharomyces Cerevisiae

ARchive for Functional Analysis
 


yeast strains


plasmids


how to order


about EUROSCARF


MIPS


SGD
The MX4blaster cassette: repeated and clean Saccharomyces cerevisiae genome modification using the genome-wide deletion collection
Angela Carvalho, Filipa Pereira & Bjorn Johansson. FEMS Yeast Res (2013) 13:711-719
new

A prototrophic deletion mutant collection for yeast metabolomics and systems biology
Mülleder M, Capuano F, Pir P, Christen S, Sauer U, Oliver SG and Ralser M  Nat Biotechnol. (2012) 30:1176-8
now
complete mutant collection available 22-02-2013

A new versatile system for rapid control of gene expression in the fission yeast Schizosaccharomyces pombe
Zilio N, Wehrkamp-Richter S and Boddy MN  Yeast (2012) 10: 425-34
new

103 plasmids deposited by EMBL scientists around the group of Jan Ellenberg
1 new plasmid available  19-10-2009
7 new plasmids available  31-08-2009
19 new plasmids available    15-01-2010
3 new plasmids available    01-02-2011

3 new plasmids available    25-08-2011
3 new plasmids available    15-01-2013

amdSYM, a recyclable and counterselectable dominant marker new
Daniel Solis-Escalante, Niels G. A. Kuijpers, Nadine Bongaerts, Irina Bolat, LizanneBosman, Jack T. Pronk, Jean-Marc Daranand Pascale Daran-Lapujade. FEMS Yeast Research (2012) doi: 10.1111/1567-1364.12024
new

Deletion marker plasmids

now all plasmid available described in:
Hegemann, J.H. and Heick, S.B. 2011. Delete and repeat: a comprehensive toolkit for sequential gene knockout in the budding yeast Saccharomyces cerevisiae. Methods Mol Biol 765, 189-206


Homoserine toxicity in Saccharomyces cerevisiae and Candida albicans homoserine kinase (thr1Delta) mutants
Joanne M. Kingsbury and John McCusker.
Eukaryot Cell (2010) 9: 717-728
new

System of centromeric, episomal, and integrative vectors based on drug resistance markers for Saccharomyces cerevisiae
Christof Taxis and Michael Knop. Biotechniques  (2006) 40, 73-77
now available

A new series of yeast shuttle vectors for the recovery and identification of multiple plasmids from Saccharomyces cerevisiae
LilyAnn Novak Frazer and Raymond T. O’Keefe. Yeast (2007) 24, 777-789
new

A fast and efficient translational control system for conditional expression of yeast genes
Kötter P, Weigand JE, Meyer B, Entian K-D, Suess B. NAR (2009) 37, e120 
new

Dominant marker vectors for selecting yeast mating products
Ivan Sadowski, Pedro Lourenco and Jennifer Parent . Yeast (2008) 25, 595-599 
new

Cytosine deaminase MX cassettes as positive/negative selectable markers in Saccharomyces cerevisiae
Phillip E. Hartzog, Bradly P. Nicholson and John H. McCusker. Yeast (2005) 22, 789-798 
new

Bimolecular fluorescence complementation analysis system for in vivo detection of protein-protein interaction in Saccharomyces cerevisiae
Min-Kyung Sung and Won-Ki Huh. Yeast (2007) 24, 767-775 
new

Vectors for N- or C-terminal positioning of the yeast Gal4p DNA binding or activator domains
Stefan H Millson, Andrew W Truman and Peter P Piper. BioTechniques (2003) 35: 60-64
new

Gene tagging and gene replacement using recombinase-mediated cassette exchange in Schizosaccharomyces pombe
Watson AT, Garcia V, Bone N, Carr AM, Armstrong J. Gene (2008) 407, 63 - 74

8 additional plasmids available

The Anchor-Away Technique: Rapid, Conditional Establishment of Yeast Mutant Phenotypes
Haruki H, Nishikawa J, Laemmli UK. Mol Cell (2008) 31, 925 - 932
Now all strains available listed in table S2 from Haruki et. (2008) 26-12-2010


An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells
T Bürckstümmer, KL Bennett, A Preradovic, G Schütze, O Hantschel, G Superti-Furga, A Bauch. Nature Methods (2006) 3, 1013-1019

An improved tetO promoter replacement system for regulating the expression of yeast genes
Yen K, Gitsham P, Wishart J, Oliver SG, Zhang N. Yeast (2003) 20, 1255 - 1262

Disintegrator vectors for single-copy yeast chromosomal integration
Ivan Sadowski, Ting-Cheng Su and Jennifer Parent. Yeast (2007) 24, 447 - 455


Almost complete yeast TAP fusion protein strain set available
Gavin et al., Nature (2006) 440, 631 - 636.

Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe
Pierre Hentges, Benoit Van Driessche, Lionel Tafforeau, Jean Vandenhoute and Anthony M. Carr. Yeast (2005) 22, 1013 - 1019

Additional vectors for PCR-based gene tagging in Saccharomyces cerevisiae and Schizosaccharomyces pombe using nourseothicin resistance
Benoit Van Driessche, Lionel Tafforeau, Pierre Hentges, Anthony M. Carr and Jean Vandenhoute. Yeast (2005) 22, 1061 - 1068

Drag & Drop Cloning in Yeast.
Gregor Jansen, Cunle Wu, Babette Schade, David Y. Thomas, Malcolm Whiteway. Gene (2005) 344, 43 - 51

New modules for the repeated internal and N-terminal epitope tagging of genes in Saccharomyces cerevisiae
Robert Gauss, Mark Trautwein, Thomas Sommer, Anne Spang. Yeast (2005) 22, 1 - 12

A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes
Carsten Janke, Maria M. Magiera, Nicole Rathfelder, Christof Taxis, Simone Reber, Hiromi Maekawa, Alexandra Moreno-Borchart, Georg Doenges, Etienne Schwob, Elmar Schiebel, Michael Knop. Yeast (2004) 21, 974 - 962

Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae
Mark A. Sheff and Kurt S. Thorn. Yeast (2004) 21, 661 - 670

LYS5 marker cassettes
Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae.
Sayoko Ito-Harashima and John McCusker. Yeast (2004) 21, 53 - 61


DsdA (D-serine deaminase): a new heterologous MX cassette of gene disruption and selection in Saccharomyces cerevisiae
Mara K. Vorachek-Warren and John McCusker. Yeast (2004) 21, 163 - 171

588 Yeast TAP fusion protein strains and TAP plasmids available
Functional organization of the yeast proteome by systematic analysis of protein complexes.
Gavin et al., Nature (2002) 415, 141-147.


Kit for making CUP1 t.s. degrons by PCR
Kanamaki, M., Sanchez-Diaz, A., Gambus, A., and Labib, K., Functional proteomic identification of DNA replication proteins by induced proteolysis in vivo. Nature (2003) 423, 720 - 724.

New: One hundred finished yeast degron strains available.

 
home    yeast strains   plasmids   how to order   about EUROSCARF   order form  

 
14-February-2014
Peter Kötter